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Anti-V5 tag Antibody (1D3)
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Catalog No. TMAC-04214
Alias V 5 tag
Anti-V5 tag Antibody (1D3) is a Mouse antibody targeting V5 tag. Anti-V5 tag Antibody (1D3) can be used in WB,IHC,IF,IP.
Anti-V5 tag Antibody (1D3)
Anti-V5 tag Antibody (1D3)
😃Good
Catalog No. TMAC-04214Alias V 5 tag
Anti-V5 tag Antibody (1D3) is a Mouse antibody targeting V5 tag. Anti-V5 tag Antibody (1D3) can be used in WB,IHC,IF,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $218 | 7-10 days | |
| 100 μL | $316 | 7-10 days |
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Product Introduction
Bioactivity
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Antigen Details
Chemical Properties
| Description | Anti-V5 tag Antibody (1D3) is a Mouse antibody targeting V5 tag. Anti-V5 tag Antibody (1D3) can be used in WB,IHC,IF,IP. |
| Synonyms | V 5 tag |
| Ig Type | IgG1 |
| Verified Activity | 1. All lanes: V5-tag mouse monoclonal antibody, A: HEK 293 cell lysate transfected without V5-tag protein, B: HEK 293 cell lysate transfected with V5 -tag protein, C: IP (HEK 293 cell without transfected + anti- V5 mAb 4℃ overnight), D: IP (PBS + anti- V5 mAb 4℃ overnight), E: IP (HEK293 transfected+protein G+anti- V5 mAb 4℃ overnight), F: IP (HEK293 transfected+RIPA+Protein G 4℃ overnight), G: Multi Tag Recombinant protein (expressed in E.coli) TP=Target Protein HC=Heavy chain LC=Light Chain. 2. IF analysis of 293 cells transfected with a V5-tag protein, using anti-V5Tag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-V5). 3. IP antibody use：5ug V5 Mouse IgG1 per ml Lysate，WB 1:5000 1、untransfected 293 cell lysate 2、transfected 293 cell lysate with V5-tag fusion protein 3、IP(untransfted 293+anti-V5 mAb+Protein G agarose) 4、IP (transfected 293+ normal Mouse IgG+Protein G agarose) 5、IP (transfected 293+anti-V5 mAb+ Protein G agarose) 6、IP (transfected 293+Protein G) 7、Recombinant protein (E.coli). |
| Application | |
| Recommended Dose | WB: 1:3000-10000; IHC: 1:200 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Background: The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). |
| Conjucates | Unconjugated |
| Immunogen | KLH conjugated synthetic peptide: GKPIPNPLLGLDST |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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