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Anti-V5 tag Antibody (1D3) (Synonyms: V 5 tag)

Catalog No. TMAC-04214 Copy Product Info
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Anti-V5 tag Antibody (1D3) is a Mouse antibody targeting V5 tag. Anti-V5 tag Antibody (1D3) can be used in IF,IHC,IP,WB.

Anti-V5 tag Antibody (1D3)

Copy Product Info
🥰Excellent
Catalog No. TMAC-04214
Synonyms V 5 tag

Anti-V5 tag Antibody (1D3) is a Mouse antibody targeting V5 tag. Anti-V5 tag Antibody (1D3) can be used in IF,IHC,IP,WB.

Anti-V5 tag Antibody
(1D3)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2187-10 days7-10 days
100 μL$3167-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-V5 tag Antibody (1D3) is a Mouse antibody targeting V5 tag. Anti-V5 tag Antibody (1D3) can be used in IF,IHC,IP,WB.
SynonymsV 5 tag
Ig Type
IgG1
Clone
1D3
Verified Activity
1. All lanes: V5-tag mouse monoclonal antibody, A: HEK 293 cell lysate transfected without V5-tag protein, B: HEK 293 cell lysate transfected with V5 -tag protein, C: IP (HEK 293 cell without transfected + anti- V5 mAb 4℃ overnight), D: IP (PBS + anti- V5 mAb 4℃ overnight), E: IP (HEK293 transfected+protein G+anti- V5 mAb 4℃ overnight), F: IP (HEK293 transfected+RIPA+Protein G 4℃ overnight), G: Multi Tag Recombinant protein (expressed in E.coli) TP=Target Protein HC=Heavy chain LC=Light Chain.
2. IF analysis of 293 cells transfected with a V5-tag protein, using anti-V5Tag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-V5).
3. IP antibody use:5ug V5 Mouse IgG1 per ml Lysate,WB 1:5000 1、untransfected 293 cell lysate 2、transfected 293 cell lysate with V5-tag fusion protein 3、IP(untransfted 293+anti-V5 mAb+Protein G agarose) 4、IP (transfected 293+ normal Mouse IgG+Protein G agarose) 5、IP (transfected 293+anti-V5 mAb+ Protein G agarose) 6、IP (transfected 293+Protein G) 7、Recombinant protein (E.coli).
verifiedActivityverifiedActivityverifiedActivity
Application
Recommended Dose
WB: 1:3000-10000; IHC: 1:200
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionHybridoma Monoclonal Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationMouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundBackground: The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5).
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
KLH conjugated synthetic peptide: GKPIPNPLLGLDST
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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