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Anti-USP28 Polyclonal Antibody

Catalog No. TMAB-14021 Copy Product Info
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Anti-USP28 Polyclonal Antibody is a Rabbit antibody targeting USP28. Anti-USP28 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-USP28 Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-14021

Anti-USP28 Polyclonal Antibody is a Rabbit antibody targeting USP28. Anti-USP28 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-USP28 Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2217-10 days7-10 days
100 μL$3737-10 days7-10 days
200 μL$5277-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-USP28 Polyclonal Antibody is a Rabbit antibody targeting USP28. Anti-USP28 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Mouse (predicted:Human,Rat)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (mouse lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (USP28) Polyclonal Antibody, Unconjugated (TMAB-14021) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Sample: Heart (Mouse) Lysate at 40 μg
Primary: Anti-Hairless (TMAB-14021) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 127 kD
Observed band size: 127 kD
Application
WB
IHC-P
IHC-Fr
IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundDeubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human USP28
Antigen Species
Human
Gene Name
USP28
Gene ID
57646
Protein Name
Ubiquitin carboxyl-terminal hydrolase 28
Uniprot ID
Q96RU2
Chemical Properties
Molecular WeightTheoretical: 75/100 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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