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Anti-USP14 Antibody (2A615)
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Catalog No. TMAC-04204
Alias USP 14, Ubiquitin-specific-processing protease 14, Ubiquitin thioesterase 14, Ubiquitin carboxyl-terminal hydrolase 14, TGT, EC 3.4.19.12, Deubiquitinating enzyme 14
Anti-USP14 Antibody (2A615) is a Rabbit antibody targeting USP14. Anti-USP14 Antibody (2A615) can be used in WB,ICC,IF,IHC,FCM.
Anti-USP14 Antibody (2A615)
Anti-USP14 Antibody (2A615)
😃Good
Catalog No. TMAC-04204Alias USP 14, Ubiquitin-specific-processing protease 14, Ubiquitin thioesterase 14, Ubiquitin carboxyl-terminal hydrolase 14, TGT, EC 3.4.19.12, Deubiquitinating enzyme 14
Anti-USP14 Antibody (2A615) is a Rabbit antibody targeting USP14. Anti-USP14 Antibody (2A615) can be used in WB,ICC,IF,IHC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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Chemical Properties
| Description | Anti-USP14 Antibody (2A615) is a Rabbit antibody targeting USP14. Anti-USP14 Antibody (2A615) can be used in WB,ICC,IF,IHC,FCM. |
| Synonyms | USP 14, Ubiquitin-specific-processing protease 14, Ubiquitin thioesterase 14, Ubiquitin carboxyl-terminal hydrolase 14, TGT, EC 3.4.19.12, Deubiquitinating enzyme 14 |
| Ig Type | IgG |
| Clone | 2A615 |
| Reactivity | Human,zebrafish |
| Verified Activity | 1. Western blot analysis of USP14 on K562 (1) and Hela (2) cell lysate using anti-USP14 antibody at 1/1,000 dilution. 2. Western blot analysis of USP14 on Zebrafish tissue lysates using anti-USP14 antibody at 1/200 dilution. 3. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-USP14 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-USP14 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-USP14 antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-USP14 antibody. Counter stained with hematoxylin. 7. ICC staining USP14 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining USP14 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. ICC staining USP14 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 10. Flow cytometric analysis of Jurkat cells with USP14 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP14 (ubiquitin specific peptidase 14), also known as TGT (tRNA-guanine transglycosylase), is a cytoplasmic protein that belongs to the ubiquitin-specific processing family of deubiquitinating enzymes. Existing as a homodimer within the cell, USP14 functions to cleave ubiquitin residues from both ubiquitinylated proteins and ubiquitin-fused precursors, thereby saving these proteins from proteasomal degradation. In mice, defects or mutations in the gene encoding USP14 cause retarded growth or fetal death, indicating that USP14 plays a key role in early developmental processes. Multiple isoforms of USP14 are expressed due to alternative splicing events. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 56 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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