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Anti-UQCRFS1 Antibody (8G153) is a Rabbit antibody targeting UQCRFS1. Anti-UQCRFS1 Antibody (8G153) can be used in WB,ICC,IF,IHC,FCM,IP.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $296 | 7-10 days | |
100 μL | $496 | 7-10 days |
Description | Anti-UQCRFS1 Antibody (8G153) is a Rabbit antibody targeting UQCRFS1. Anti-UQCRFS1 Antibody (8G153) can be used in WB,ICC,IF,IHC,FCM,IP. |
Synonyms | UQCRFS 1, UQCR5, UCRI_HUMAN, Ubiquinol-cytochrome c reductase iron-sulfur subunit, Ubiquinol-cytochrome c reductase 8 kDa protein, Ubiquinol cytochrome c reductase Rieske iron-sulfur polypeptide 1, Ubiquinol cytochrome C reductase rieske iron sulphur, RISP, RIS1, RIP1, Rieske iron-sulfur protein, Rieske iron sulfur protein, Proton gradient regulation protein 1, Plastohydroquinone:plastocyanin oxidoreductase iron sulfur protein, PGR1, petC, Cytochrome b-c1 complex subunit 5, Cytochrome b-c1 complex subunit 11, Cytochrome b6f complex iron sulfur subunit, chloroplastic, Cytochrome b c1 complex subunit Rieske mitochondrial, Complex III subunit IX, Complex III subunit 5 |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Western blot analysis of UQCRFS1 on different lysates using anti-UQCRFS1 antibody at 1/1,000 dilution. Positive control: Lane 1: A549, Lane 2: 293, Lane 3: Mouse kidney. 2. Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-UQCRFS1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-UQCRFS1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-UQCRFS1 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-UQCRFS1 antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-UQCRFS1 antibody. Counter stained with hematoxylin. 7. ICC staining UQCRFS1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining UQCRFS1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. ICC staining UQCRFS1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 10. Flow cytometric analysis of A549 cells with UQCRFS1 antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Application | |
Recommended Dose | WB: 1:500-2000; IHC: 1:50-200; ICC: 1:500-2000; FCM: 1:10-50 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | Recombinant Antibody |
Purification | ProA affinity purified |
Appearance | Liquid |
Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
Research Background | Cytochrome b-c1 complex subunit Rieske, mitochondrial: Component of the mitochondrial ubiquinol-cytochrome c reductase complex dimer (complex III dimer), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis. Incorporation of UQCRFS1 is the penultimate step in complex III assembly. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein: human UQCRFS1 aa 100 to the C-terminus |
Antigen Species | human |
Uniprot ID |
Molecular Weight | Theoretical: 21 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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