Anti-uPAR/PLAUR Polyclonal Antibody is a Rabbit antibody targeting uPAR/PLAUR. Anti-uPAR/PLAUR Polyclonal Antibody can be used in IF, IHC-Fr, IHC-P, WB.

IgG

Mouse,Rat (predicted:Human,Dog,Cow,Sheep)

1. Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-uPA Receptor Polyclonal Antibody, Unconjugated (TMAB-01936) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.

2. Tissue/cell: mouse adrenal gland; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-uPA Receptor Polyclonal Antibody, Unconjugated (TMAB-01936) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Sample:
Lane 1: Cerebrum (Mouse) Tissue Lysate at 40 μg
Lane 2: Cerebrum (Rat) Tissue Lysate at 40 μg
Primary: Anti-uPA Receptor (TMAB-01936) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 48 kDa
4. Sample:
bone (Mouse) Lysate at 40 μg
Primary: Anti-uPA Receptor (TMAB-01936) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 48 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human PLAUR

Human

PLAUR

Urokinase plasminogen activator surface receptor

Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form.