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Anti-uPAR/PLAUR Polyclonal Antibody

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Catalog No. TMAB-01936

Anti-uPAR/PLAUR Polyclonal Antibody is a Rabbit antibody targeting uPAR/PLAUR. Anti-uPAR/PLAUR Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-uPAR/PLAUR Polyclonal Antibody

Anti-uPAR/PLAUR Polyclonal Antibody

😃Good
Catalog No. TMAB-01936
Anti-uPAR/PLAUR Polyclonal Antibody is a Rabbit antibody targeting uPAR/PLAUR. Anti-uPAR/PLAUR Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$221 7-10 days
100 μL$373 7-10 days
200 μL$528 7-10 days
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Product Introduction

Bioactivity
Description
Anti-uPAR/PLAUR Polyclonal Antibody is a Rabbit antibody targeting uPAR/PLAUR. Anti-uPAR/PLAUR Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Dog,Cow,Sheep)
Verified Activity
1. Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-uPA Receptor Polyclonal Antibody, Unconjugated (TMAB-01936) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.

2. Tissue/cell: mouse adrenal gland; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-uPA Receptor Polyclonal Antibody, Unconjugated (TMAB-01936) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Sample:
Lane 1: Cerebrum (Mouse) Tissue Lysate at 40 μg
Lane 2: Cerebrum (Rat) Tissue Lysate at 40 μg
Primary: Anti-uPA Receptor (TMAB-01936) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 48 kDa
4. Sample:
bone (Mouse) Lysate at 40 μg
Primary: Anti-uPA Receptor (TMAB-01936) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 48 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationIsoform 1: Cell membrane; Lipid-anchor, GPI-anchor. Isoform 2: Secreted (Probable).
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes the receptor for urokinase plasminogen activator and, given its role in localizing and promoting plasmin formation, likely influences many normal and pathological processes related to cell-surface plasminogen activation and localized degradation of the extracellular matrix. It binds both the proprotein and mature forms of urokinase plasminogen activator and permits the activation of the receptor-bound pro-enzyme by plasmin. The protein lacks transmembrane or cytoplasmic domains and may be anchored to the plasma membrane by a glycosyl-phosphatidylinositol(GPI) moiety following cleavage of the nascent polypeptide near its carboxy-terminus. However, a soluble protein is also produced in some cell types. Alternative splicing results in multiple transcript variants encoding different isoforms. The proprotein experiences several post-translational cleavage reactions that have not yet been fully defined. [provided by RefSeq].
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human PLAUR
Antigen Species
Human
Gene ID
Uniprot ID
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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