Anti-UGDH Antibody (8N278) is a Mouse antibody targeting UGDH. Anti-UGDH Antibody (8N278) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

IgG1, Kappa

8N278

Human (predicted:Mouse,Rat)

1. Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for TMAB-13980
2. Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:5000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: 1: Hela, 2: HepG2, 3: Rat Liver, 4: F9
Protein loading quantity: 20 μg
Exposure time: 3 s
Predicted MW: 55 kDa
Observed MW: 55 kDa
3. Tissue: Human prostatic hyperplasia
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: SP Kit (Mouse)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-13980

WB: 1:1000-5000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:50-200; IF: 1:100-500

Monoclonal

UGDH

UDP-glucose 6-dehydrogenase

Involved in the biosynthesis of glycosaminoglycans; hyaluronan, chondroitin sulfate, and heparan sulfate.