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Anti-UCHL3 Antibody (9J995)
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Catalog No. TMAY-03404
Alias ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
Anti-UCHL3 Antibody (9J995) is a Rabbit antibody targeting UCHL3. Anti-UCHL3 Antibody (9J995) can be used in WB,IP.
Anti-UCHL3 Antibody (9J995)
Anti-UCHL3 Antibody (9J995)
😃Good
Catalog No. TMAY-03404Alias ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
Anti-UCHL3 Antibody (9J995) is a Rabbit antibody targeting UCHL3. Anti-UCHL3 Antibody (9J995) can be used in WB,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $269 | 7-10 days |
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Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-UCHL3 Antibody (9J995) is a Rabbit antibody targeting UCHL3. Anti-UCHL3 Antibody (9J995) can be used in WB,IP. |
| Alias | ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 9J995 |
| Reactivity | Mouse |
| Verified Activity | 1. Anti-UCHL3 rabbit monoclonal antibody at 1:500 dilution. -Lane A: Jurkat Whole Cell Lysate. -Lane B: K562 Whole Cell Lysate. -Lane C: 293T Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:26 kDa. -Observed band size:26 kDa(We are unsure as to the identity of these extra bands.) 2. Mouse UCHL3 was immunoprecipitated using: -Lane A:0.5 mg K562 Whole Cell Lysate. -Lane B:0.5 mg 293T Whole Cell Lysate. -2 µL anti-Mouse UCHL3 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-Mouse UCHL3 rabbit monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 26 kDa. -Observed band size: 26 kDa |
| Application | WB,IP |
| Recommended Dose | WB: 1:500-1:2000; IP: 0.5-2 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse UCHL3 (rM UCHL3; TMPY-02190; Q9JKB1; Glu2-Ala230). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Ubiquitin carboxyl-terminal hydrolase isozyme L3, also known as UCH-L3, Ubiquitin thioesterase L3 and UCHL3, is a ubiquitin-protein hydrolase that belongs to the peptidase C12 family. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. UCHL3 is highly expressed in heart, skeletal muscle, and testis. UCHL1 and UCHL3 are two of the deubiquitinating enzymes expressed in the brain. These phenotypes indicate the importance of UCHL1 and UCHL3 in the regulation of the central nervous system. UCHL3 functions as a de-ubiquitinating enzyme where lack of its hydrolase activity may result in the prominent accumulation of ubiquitinated proteins and subsequent induction of stress responses in skeletal muscle. UCHL3 has also been identified as a tumor-specific antigen in colon cancer. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Mouse UCHL3 Protein (TMPY-02190) |
| Antigen Species | Mouse |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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