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Anti-Ubiquityl-Histone H2B (Lys120) Antibody (4B373)
Catalog No. TMAB-13957 Copy Product Info
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Anti-Ubiquityl-Histone H2B (Lys120) Antibody (4B373) is a Mouse antibody targeting Ubiquityl-Histone H2B (Lys120). Anti-Ubiquityl-Histone H2B (Lys120) Antibody (4B373) can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Ubiquityl-Histone H2B (Lys120) Antibody (4B373)
Copy Product Info🥰Excellent
Catalog No. TMAB-13957
Anti-Ubiquityl-Histone H2B (Lys120) Antibody (4B373) is a Mouse antibody targeting Ubiquityl-Histone H2B (Lys120). Anti-Ubiquityl-Histone H2B (Lys120) Antibody (4B373) can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Ubiquityl-Histone H2B
(Lys120) Antibody
(4B373)
(Lys120) Antibody
(4B373)
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 25 μL | $148 | 7-10 days | 7-10 days | |
| 50 μL | $263 | 7-10 days | 7-10 days | |
| 100 μL | $472 | 7-10 days | 7-10 days |
In stock · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
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| Description | Anti-Ubiquityl-Histone H2B (Lys120) Antibody (4B373) is a Mouse antibody targeting Ubiquityl-Histone H2B (Lys120). Anti-Ubiquityl-Histone H2B (Lys120) Antibody (4B373) can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG2a, Kappa |
| Clone | 4B373 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Tissue: Mouse kidney Section type: Formalin fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit (Mouse) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for TMAB-13957 2. Tissue: Rat liver Section type: Formalin fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit (Mouse) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for TMAB-13957 3. Tissue: Human liver Section type: Formalin fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit (Mouse) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for TMAB-13957 4. Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:1000 Primary ab incubation condition: 4°C overnight Secondary ab: Goat Anti-Mouse IgG H&L (HRP) Lysate: HeLa, HepG2, Neuro-2a Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 23 kDa Observed MW: 23 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Nucleus. Chromosome. |
| Construction | Recombinant Antibody |
| Purification | Antigen affinity purification |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]. |
| Gene Name | H2BC3 |
| Gene ID | |
| Protein Name | Histone H2B type 1-B |
| Function | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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