Anti-Ubiquityl-Histone H2B (Lys120) Antibody (2R144) is a Rabbit antibody targeting Ubiquityl-Histone H2B (Lys120). Anti-Ubiquityl-Histone H2B (Lys120) Antibody (2R144) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

2R144

Human,Mouse,Rat

1. Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1: 2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: HeLa, N2a, BRL, Mouse liver, Mouse kidney
Protein loading quantity: 20 μg
Exposure time: 30
Predicted MW: 23 Da
Observed MW: 23 Da
2. Tissue: Mouse liver
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit (Rabbit)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-13958
3. Tissue: Human lung squamous carcinoma
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit (Rabbit)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-13958
4. Tissue: Rat liver
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit (Rabbit)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-13958

WB: 1:500-2000; IHC-P: 1:200-1000; IHC-Fr: 1:200-1000; IF: 1:200-1000

Monoclonal

KLH conjugated synthetic peptide: human Ubiquityl-Histone H2B (Lys120)

Human

H2BC3

Histone H2B type 1-B

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.