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Anti-Ubiquitin D Antibody (7G418)
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Catalog No. TMAC-04189
Alias Ubiquitin-like protein FAT10, UbiquitinD, Ubiquitin like protein FAT10, UBD_HUMAN, UBD 3, Ubd, GABBR1, FAT10, Diubiquitin
Anti-Ubiquitin D Antibody (7G418) is a Rabbit antibody targeting Ubiquitin D. Anti-Ubiquitin D Antibody (7G418) can be used in WB,ICC,IHC.
Anti-Ubiquitin D Antibody (7G418)
Anti-Ubiquitin D Antibody (7G418)
😃Good
Catalog No. TMAC-04189Alias Ubiquitin-like protein FAT10, UbiquitinD, Ubiquitin like protein FAT10, UBD_HUMAN, UBD 3, Ubd, GABBR1, FAT10, Diubiquitin
Anti-Ubiquitin D Antibody (7G418) is a Rabbit antibody targeting Ubiquitin D. Anti-Ubiquitin D Antibody (7G418) can be used in WB,ICC,IHC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
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Chemical Properties
| Description | Anti-Ubiquitin D Antibody (7G418) is a Rabbit antibody targeting Ubiquitin D. Anti-Ubiquitin D Antibody (7G418) can be used in WB,ICC,IHC. |
| Synonyms | Ubiquitin-like protein FAT10, UbiquitinD, Ubiquitin like protein FAT10, UBD_HUMAN, UBD 3, Ubd, GABBR1, FAT10, Diubiquitin |
| Ig Type | IgG |
| Clone | 7G418 |
| Reactivity | Human,Mouse |
| Verified Activity | 1. Western blot analysis of Ubiquitin D on Hela cells lysates using anti-Ubiquitin D antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Ubiquitin D antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Ubiquitin D antibody. Counter stained with hematoxylin. 4. ICC staining Ubiquitin D in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining Ubiquitin D in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining Ubiquitin D in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | FAT10, also designated Ubiquitin D or Diubiquitin, is a 165 amino acid protein encoded in the major histocompatibility complex (MHC) that consists of two domains which share significant homology with ubiquitin. Each domain contains two cysteines, along with a free C-terminal diglycine motif required for FAT10 conjugate formation. FAT10 is inducible by interferon-g and tumor necrosis factor a (TNF?). The FAT10 protein interacts with MAD2, a component of the spindle checkpoint, and plays a role in antigen presentation, cytokine response, apoptosis and mitosis. It may also regulate cell growth during dendritic cell or B cell activation and development. FAT10 mRNA is expressed mainly in some dendritic cells and lymphoblastoid lines and in other specific cells subsequent to interferon-g induction. The human FAT10 gene, designated UBD, maps to chromosome 6p21.3 and is overexpressed in the tumors of various epithelial cancers. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 18 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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