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Anti-Ubiquitin Activating Enzyme E1/UBA1 Antibody (1V154)

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Catalog No. TMAC-04193
Alias ubiquitin-like modifier activating enzyme 1, UBE1X, UBE1, UBA1A, SMAX2, POC20, GXP1, CTD-2522E6.1, CFAP124, AMCX1, A1ST, A1S9T, A1S9

Anti-Ubiquitin Activating Enzyme E1/UBA1 Antibody (1V154) is a Rabbit antibody targeting Ubiquitin Activating Enzyme E1/UBA1. Anti-Ubiquitin Activating Enzyme E1/UBA1 Antibody (1V154) can be used in WB,ICC/IF,IHC,FCM.

Anti-Ubiquitin Activating Enzyme E1/UBA1 Antibody (1V154)

Anti-Ubiquitin Activating Enzyme E1/UBA1 Antibody (1V154)

😃Good
Catalog No. TMAC-04193Alias ubiquitin-like modifier activating enzyme 1, UBE1X, UBE1, UBA1A, SMAX2, POC20, GXP1, CTD-2522E6.1, CFAP124, AMCX1, A1ST, A1S9T, A1S9
Anti-Ubiquitin Activating Enzyme E1/UBA1 Antibody (1V154) is a Rabbit antibody targeting Ubiquitin Activating Enzyme E1/UBA1. Anti-Ubiquitin Activating Enzyme E1/UBA1 Antibody (1V154) can be used in WB,ICC/IF,IHC,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2977-10 days
100 μL$4977-10 days
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Product Introduction

Bioactivity
Description
Anti-Ubiquitin Activating Enzyme E1/UBA1 Antibody (1V154) is a Rabbit antibody targeting Ubiquitin Activating Enzyme E1/UBA1. Anti-Ubiquitin Activating Enzyme E1/UBA1 Antibody (1V154) can be used in WB,ICC/IF,IHC,FCM.
Synonymsubiquitin-like modifier activating enzyme 1, UBE1X, UBE1, UBA1A, SMAX2, POC20, GXP1, CTD-2522E6.1, CFAP124, AMCX1, A1ST, A1S9T, A1S9
Ig Type
IgG
Clone
1V154
Reactivity
Human,Mouse,Rat
Verified Activity
1. Western blot analysis of UBA1 on mouse liver lysates using anti-UBA1 antibody at 1/5,000 dilution.
2. Immunohistochemical analysis of paraffin-embedded mouse ovary tissue using anti-UBA1 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-UBA1 antibody. Counter stained with hematoxylin.
4. ICC staining UBA1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. ICC staining UBA1 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. ICC staining UBA1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. Flow cytometric analysis of K562 cells with UBA1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Application
Recommended Dose
WB: 1:1000-2000; IHC: 1:100-500; ICC/IF: 1:50-200; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundThe ubiquitin activating enzyme E1 (UBE1) catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. Specifically, UBE1 functions to adenylate the C-terminal glycine residue of ubiquitin, a reaction that is ATP-dependent and is proceeded by the formation of a thiolester bond with a cysteine residue of UBE1. The UBE1-activated ubiquitin is then transferred to a ubiquitin conjugated enzyme, which donates the ubiquitin residue to target substrates. The UBE1 gene is an example of an X-Y homologous gene, which is X-linked with a distinct Y-linked gene in many mammals. However, no UBE1 homolog is detectable on the human Y chromosome. UBE1 is thought to escape X inactivation in humans.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 118 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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