Anti-UBE2Q1 Polyclonal Antibody 2 is a Rabbit antibody targeting UBE2Q1. Anti-UBE2Q1 Polyclonal Antibody 2 can be used in FCM.

IgG

Mouse (predicted:Human,Rat,Dog,Cow,Horse,Rabbit,Sheep)

1. cells: Mouse splenocyte
Concentration: 2 μg/10^6 cells, 4℃ incubate 30 min.
2. Blank control: Mouse spleen.
Primary Antibody (green line): Rabbit Anti-UBE2Q1 antibody (TMAB-13942)
Dilution: 2 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

FCM: 2μg/Test

Polyclonal

KLH conjugated synthetic peptide: human UBE2Q1

Human

UBE2Q1

Ubiquitin-conjugating enzyme E2 Q1