Anti-UBE2B Antibody (4P896) is a Rabbit antibody targeting UBE2B. Anti-UBE2B Antibody (4P896) can be used in ICC/IF, IF, IHC-Fr, IHC-P, WB.

IgG

4P896

Human

25 ug total protein per lane of various lysates (see on figure) probed with UBE2B monoclonal antibody, unconjugated (TMAB-13928) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

ICC/IF=1:50-200; IF=1:50-200; IHC-Fr=1:100-200; IHC-P=1:100-200; WB=1:500-2000

Monoclonal

A synthesized peptide: human UBE2B

Human

UBE2B

Ubiquitin-conjugating enzyme E2 B

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth.