Anti-Tyrosinase/TYR Polyclonal Antibody is a Rabbit antibody targeting Tyrosinase/TYR. Anti-Tyrosinase/TYR Polyclonal Antibody can be used in IF, IHC-Fr, IHC-P, WB.

IgG

Mouse,Rat (predicted:Human,Dog,Pig,Cow,Sheep)

1. Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-yrosinase Polyclonal Antibody, Unconjugated (TMAB-01923) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample:
Lane 1: Eye (Mouse) Lysate at 40 μg
Lane 2: Skin (Mouse) Lysate at 40 μg
Primary: Anti-Tyrosinase (TMAB-01923) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80 kDa
Observed band size: 80 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Tyrosinase

Human

TYR

Tyrosinase

This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone.