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Anti-Tyrosinase/TYR Polyclonal Antibody
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Catalog No. TMAB-01923
Alias Tyrosinase, TYR, Tumor rejection antigen AB, SK29-AB, Monophenol monooxygenase, LB24-AB
Anti-Tyrosinase/TYR Polyclonal Antibody is a Rabbit antibody targeting Tyrosinase/TYR. Anti-Tyrosinase/TYR Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Tyrosinase/TYR Polyclonal Antibody
Anti-Tyrosinase/TYR Polyclonal Antibody
😃Good
Catalog No. TMAB-01923Alias Tyrosinase, TYR, Tumor rejection antigen AB, SK29-AB, Monophenol monooxygenase, LB24-AB
Anti-Tyrosinase/TYR Polyclonal Antibody is a Rabbit antibody targeting Tyrosinase/TYR. Anti-Tyrosinase/TYR Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Tyrosinase/TYR Polyclonal Antibody is a Rabbit antibody targeting Tyrosinase/TYR. Anti-Tyrosinase/TYR Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | Tyrosinase, TYR, Tumor rejection antigen AB, SK29-AB, Monophenol monooxygenase, LB24-AB |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Cow,Sheep) |
| Verified Activity | 1. Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-yrosinase Polyclonal Antibody, Unconjugated (TMAB-01923) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Sample: Lane 1: Eye (Mouse) Lysate at 40 μg Lane 2: Skin (Mouse) Lysate at 40 μg Primary: Anti-Tyrosinase (TMAB-01923) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 80 kDa Observed band size: 80 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Melanosome membrane; Single-pass type I membrane protein. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Tyrosinase is the key enzyme for melanin,synthesis in mammalian melanocytes and has been considered to be a unique marker for the study of melanocyte differentiation. A cDNA library was constructed from poly(A)+ mRNA from mouse melanocytes and screened using anti- tyrosinase antiserum and oligonucleotide probes corresponding to amino acid sequence of tyrosinase. sequencing of some cDNA clones positive in these screenings gave a nucleotide sequence of 1838 nucleotides including a open reading frame of 1344 nucleotides that was found to correspond exactly to the amino acid sequence of the cyanogen bromide fragments of tyrosinase. |
| Immunogen | KLH conjugated synthetic peptide: human Tyrosinase |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID |
| Molecular Weight | Theoretical: 56 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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