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Anti-TYMS Polyclonal Antibody
Catalog No. TMAB-13909 Copy Product Info
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Anti-TYMS Polyclonal Antibody is a Rabbit antibody targeting TYMS. Anti-TYMS Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-TYMS Polyclonal Antibody
Copy Product Info🥰Excellent
Catalog No. TMAB-13909
Anti-TYMS Polyclonal Antibody is a Rabbit antibody targeting TYMS. Anti-TYMS Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-TYMS Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $223 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-TYMS Polyclonal Antibody is a Rabbit antibody targeting TYMS. Anti-TYMS Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Pig,Cow,Rabbit,Sheep) |
| Verified Activity | 1. Sample: Bone (Mouse) Lysate at 40 μg Primary: Anti-Thymidylate synthase (TMAB-13909) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 38 kD 2. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Thymidylate synthase) Polyclonal Antibody, Unconjugated (TMAB-13909) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. Cytoplasm. Mitochondrion. Mitochondrion matrix. Mitochondrion inner membrane. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using, 10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occurring antisense transcript, mitochondrial enolase superfamily member 1 (GeneID:55556), vary inversely when cell-growth progresses from late-log to plateau phase. Polymorphisms in this gene may be associated with etiology of neoplasia, including breast cancer, and response to chemotherapy. [provided by RefSeq, Aug 2017] |
| Immunogen | KLH conjugated synthetic peptide: human TYMS |
| Antigen Species | Human |
| Gene Name | TYMS |
| Gene ID | |
| Protein Name | Thymidylate synthase |
| Uniprot ID | |
| Function | Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. |
| Molecular Weight | Theoretical: 36 kDa. Actual: 38 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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