Anti-TXNRD1 Antibody (2O425) is a Rabbit antibody targeting TXNRD1. Anti-TXNRD1 Antibody (2O425) can be used in FCM, ICC/IF, IF, IHC-Fr, IHC-P, IP, WB.

IgG

2O425

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TXNRD1 Monoclonal Antibody, Unconjugated (TMAB-13906) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TXNRD1 Monoclonal Antibody, Unconjugated (TMAB-13906) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TXNRD1 Monoclonal Antibody, Unconjugated (TMAB-13906) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Mouse Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TXNRD1 Monoclonal Antibody, Unconjugated (TMAB-13906) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Rat Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TXNRD1 Monoclonal Antibody, Unconjugated (TMAB-13906) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TXNRD1 Monoclonal Antibody, Unconjugated (TMAB-13906) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TXNRD1 Monoclonal Antibody, Unconjugated (TMAB-13906) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancert; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TXNRD1 Monoclonal Antibody, Unconjugated (TMAB-13906) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Human Prostate tumors; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TXNRD1 Monoclonal Antibody, Unconjugated (TMAB-13906) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TXNRD1 Monoclonal Antibody, Unconjugated (TMAB-13906) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
11. 25 ug total protein per lane of various lysates (see on figure) probed with TXNRD1 monoclonal antibody, unconjugated (TMAB-13906) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
12. 25 ug total protein per lane of various lysates (see on figure) probed with TXNRD1 monoclonal antibody, unconjugated (TMAB-13906) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
13. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TXNRD1 Monoclonal Antibody, Unconjugated (TMAB-13906) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
14. The A549 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-TXNRD1 antibody (TMAB-13906): 1 μg/10^6 cells; Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
15. 4% Paraformaldehyde-fixed A549 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (TXNRD1) monoclonal Antibody, unconjugated (TMAB-13906) 1: 100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, BF488) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

FCM=1 μg/Test; ICC/IF=1:50-200; IF=1:100-500; IHC-Fr=1:100-500; IHC-P=1:100-500; IP=1:20-50; WB=1:1000-5000

Monoclonal

A synthesized peptide: human TR

Human

TXNRD1

Thioredoxin reductase 1, cytoplasmic