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Anti-Twist Antibody (5E952)

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Catalog No. TMAC-04169

Anti-Twist Antibody (5E952) is a Mouse antibody targeting Twist. Anti-Twist Antibody (5E952) can be used in WB,IP,IF,FCM.

Anti-Twist Antibody (5E952)

Anti-Twist Antibody (5E952)

😃Good
Catalog No. TMAC-04169
Anti-Twist Antibody (5E952) is a Mouse antibody targeting Twist. Anti-Twist Antibody (5E952) can be used in WB,IP,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2987-10 days
100 μL$4277-10 days
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Product Introduction

Bioactivity
Description
Anti-Twist Antibody (5E952) is a Mouse antibody targeting Twist. Anti-Twist Antibody (5E952) can be used in WB,IP,IF,FCM.
Reactivity
Human,Mouse,Rat
Application
Recommended Dose
WB: 1:100-1000; IP: 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate); FCM: 1 μg per 1 x 106 cells
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionHybridoma Monoclonal Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundMembers of the myogenic determination family are basic helix-loop-helix (bHLH) proteins that can be separated into two classes. Class A proteins include the ubiquitously expressed E-box binding factors E12/E47, ITF2 and HEB (BETA1 or HTF4). Class B proteins such as MyoD, myogenin and NeuroD (BETA2) are transiently expressed and exhibit a much more limited tissue distribution. Class A proteins heterodimerize with class B proteins to activate DNA transcription. Working in opposition to these positively acting factors are a specialized group of proteins that function as dominant negative regulators. Muscle tissue is derived from a subset of cells originating from the embryonic mesoderm. The novel basic helix-loop-helix (bHLH) transcription factor twist is a putative regulator of mesodermal differentiation and myogenesis. Twist is expressed throughout the epithelial somite but not in the myotome. Twist requires dimerization with the E proteins and inhibits myogenic regulatory factors. It has been implicated as regulator of the temporal and spatial formation of myotomes.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein: a region near the C-terminus of twist of human origin
Antigen Species
human
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 28 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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