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Anti-Twist Antibody (5E952)
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Catalog No. TMAC-04169
Anti-Twist Antibody (5E952) is a Mouse antibody targeting Twist. Anti-Twist Antibody (5E952) can be used in WB,IP,IF,FCM.
Anti-Twist Antibody (5E952)
Anti-Twist Antibody (5E952)
😃Good
Catalog No. TMAC-04169
Anti-Twist Antibody (5E952) is a Mouse antibody targeting Twist. Anti-Twist Antibody (5E952) can be used in WB,IP,IF,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $427 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-Twist Antibody (5E952) is a Mouse antibody targeting Twist. Anti-Twist Antibody (5E952) can be used in WB,IP,IF,FCM. |
| Reactivity | Human,Mouse,Rat |
| Application | |
| Recommended Dose | WB: 1:100-1000; IP: 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate); FCM: 1 μg per 1 x 106 cells |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Members of the myogenic determination family are basic helix-loop-helix (bHLH) proteins that can be separated into two classes. Class A proteins include the ubiquitously expressed E-box binding factors E12/E47, ITF2 and HEB (BETA1 or HTF4). Class B proteins such as MyoD, myogenin and NeuroD (BETA2) are transiently expressed and exhibit a much more limited tissue distribution. Class A proteins heterodimerize with class B proteins to activate DNA transcription. Working in opposition to these positively acting factors are a specialized group of proteins that function as dominant negative regulators. Muscle tissue is derived from a subset of cells originating from the embryonic mesoderm. The novel basic helix-loop-helix (bHLH) transcription factor twist is a putative regulator of mesodermal differentiation and myogenesis. Twist is expressed throughout the epithelial somite but not in the myotome. Twist requires dimerization with the E proteins and inhibits myogenic regulatory factors. It has been implicated as regulator of the temporal and spatial formation of myotomes. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: a region near the C-terminus of twist of human origin |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Theoretical: 28 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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