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Anti-TUBB3 Antibody (5D159)

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Catalog No. TMAC-04163
Alias Tubulin beta-III, Tubulin beta-4 chain, Tubulin beta-3 chain, TUBB4, TUBB3

Anti-TUBB3 Antibody (5D159) is a Rabbit antibody targeting TUBB3. Anti-TUBB3 Antibody (5D159) can be used in WB,IHC,IP,FCM.

Anti-TUBB3 Antibody (5D159)

Anti-TUBB3 Antibody (5D159)

😃Good
Catalog No. TMAC-04163Alias Tubulin beta-III, Tubulin beta-4 chain, Tubulin beta-3 chain, TUBB4, TUBB3
Anti-TUBB3 Antibody (5D159) is a Rabbit antibody targeting TUBB3. Anti-TUBB3 Antibody (5D159) can be used in WB,IHC,IP,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2987-10 days
100 μL$4977-10 days
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Product Introduction

Bioactivity
Description
Anti-TUBB3 Antibody (5D159) is a Rabbit antibody targeting TUBB3. Anti-TUBB3 Antibody (5D159) can be used in WB,IHC,IP,FCM.
SynonymsTubulin beta-III, Tubulin beta-4 chain, Tubulin beta-3 chain, TUBB4, TUBB3
Ig Type
IgG
Clone
5D159
Reactivity
Human,Mouse,Rat
Verified Activity
1. Western blot analysis of Tubulin beta-III on different lysates using anti-Tubulin beta-III antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: PC-12, Lane 3: SH-SY-5Y.
2. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Tubulin beta-III antibody. Counter stained with hematoxylin.
3. Flow cytometric analysis of N2A cells with Tubulin beta-III antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Application
Recommended Dose
WB: 1:1000-2000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundTubulin is a major cytoskeleton component that has five distinct forms, designated α, β, γ, δ and e Tubulin. α and β Tubulins form heterodimers which multimerize to form a microtubule filament. Multiple β Tubulin isoforms (β1, β2, β3, β4, β5, β6 and β8) have been characterized and are expressed in mammalian tissues. β1 and β4 are present throughout the cytosol, β2 is present in the nuclei and nucleoplasm, and β3 is a neuron-specific cytoskeletal protein. γ Tubulin forms the gammasome, which is required for nucleating microtubule filaments at the centrosome. Both δ Tubulin and e Tubulin are associated with the centrosome. δ Tubulin is a homolog of the Chlamydomonas δ Tubulin Uni3 and is found in association with the centrioles, whereas e Tubulin localizes to the pericentriolar material. e Tubulin exhibits a cell-cycle-specific pattern of localization, first associating with only the older of the centrosomes in a newly duplicated pair and later associating with both centrosomes.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 50 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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