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Anti-TUBB Antibody (8X869)
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Catalog No. TMAC-04342
Alias Tubulin beta-5 chain, Tubulin beta chain, TUBB5, TUBB
Anti-TUBB Antibody (8X869) is a Mouse antibody targeting TUBB. Anti-TUBB Antibody (8X869) can be used in FCM,ICC,IHC,WB.
Anti-TUBB Antibody (8X869)
Anti-TUBB Antibody (8X869)
😃Good
Catalog No. TMAC-04342Alias Tubulin beta-5 chain, Tubulin beta chain, TUBB5, TUBB
Anti-TUBB Antibody (8X869) is a Mouse antibody targeting TUBB. Anti-TUBB Antibody (8X869) can be used in FCM,ICC,IHC,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $427 | 7-10 days |
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Chemical Properties
| Description | Anti-TUBB Antibody (8X869) is a Mouse antibody targeting TUBB. Anti-TUBB Antibody (8X869) can be used in FCM,ICC,IHC,WB. |
| Synonyms | Tubulin beta-5 chain, Tubulin beta chain, TUBB5, TUBB |
| Clone | 8X869 |
| Reactivity | Human,Mouse,Rat,zebrafish |
| Verified Activity | 1. Western blot analysis of β-tubulin on different cell lysates using anti-β-tubulin antibody at 1/5000 dilution. Positive control: Lane 1: NCCIT, Lane 2: NIH/3T3, Lane 3: PC12, Lane 4: Mouse heart, Lane 5: F9, Lane 6: zebrafish brain, Lane 7: Hela. 2. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-β-tubulin antibody. Counter stained with hematoxylin. 3. ICC staining β-tubulin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC stainingβ-tubulin in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining:-tubulin in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. Flow cytometric analysis of HeLa?cells with β-tubulin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.       |
| Application | |
| Recommended Dose | WB: 1:5000-10000; IHC: 1:20; ICC: 1:200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin. The beta-tubulin (relative molecular weight about 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain. Beta-Tubulin may have bound GTP or GDP. Under certain conditions β-tubulin can hydrolyze its bound GTP to GDP plus Pi, release the Pi, and exchange the GDP for GTP. |
| Conjucates | Unconjugated |
| Immunogen | Peptide |
| Uniprot ID |
| Molecular Weight | Theoretical: 50 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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