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Anti-TUBB Antibody (8X869) (Synonyms: Tubulin beta-5 chain, Tubulin beta chain, TUBB5, TUBB)

Catalog No. TMAC-04342 Copy Product Info
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Anti-TUBB Antibody (8X869) is a Mouse antibody targeting TUBB. Anti-TUBB Antibody (8X869) can be used in FCM,ICC,IHC,WB.

Anti-TUBB Antibody (8X869)

Copy Product Info
🥰Excellent
Catalog No. TMAC-04342
Synonyms Tubulin beta-5 chain, Tubulin beta chain, TUBB5, TUBB

Anti-TUBB Antibody (8X869) is a Mouse antibody targeting TUBB. Anti-TUBB Antibody (8X869) can be used in FCM,ICC,IHC,WB.

Anti-TUBB Antibody
(8X869)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2977-10 days7-10 days
100 μL$4277-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-TUBB Antibody (8X869) is a Mouse antibody targeting TUBB. Anti-TUBB Antibody (8X869) can be used in FCM,ICC,IHC,WB.
SynonymsTubulin beta-5 chain, Tubulin beta chain, TUBB5, TUBB
Clone
8X869
Reactivity
Human,Mouse,Rat,zebrafish
Verified Activity
1. Western blot analysis of β-tubulin on different cell lysates using anti-β-tubulin antibody at 1/5000 dilution. Positive control: Lane 1: NCCIT, Lane 2: NIH/3T3, Lane 3: PC12, Lane 4: Mouse heart, Lane 5: F9, Lane 6: zebrafish brain, Lane 7: Hela.
2. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-β-tubulin antibody. Counter stained with hematoxylin.
3. ICC staining β-tubulin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
4. ICC stainingβ-tubulin in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. ICC staining:-tubulin in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. Flow cytometric analysis of HeLa?cells with β-tubulin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.
verifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivity
Application
Recommended Dose
WB: 1:5000-10000; IHC: 1:20; ICC: 1:200; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionHybridoma Monoclonal Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundTubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin. The beta-tubulin (relative molecular weight about 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain. Beta-Tubulin may have bound GTP or GDP. Under certain conditions β-tubulin can hydrolyze its bound GTP to GDP plus Pi, release the Pi, and exchange the GDP for GTP.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Peptide
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 50 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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