Anti-TUBB Antibody (8X869) is a Mouse antibody targeting TUBB. Anti-TUBB Antibody (8X869) can be used in WB,ICC,IHC,FCM.

Human,Mouse,Rat,zebrafish

1. Western blot analysis of β-tubulin on different cell lysates using anti-β-tubulin antibody at 1/5000 dilution. Positive control: Lane 1: NCCIT, Lane 2: NIH/3T3, Lane 3: PC12, Lane 4: Mouse heart, Lane 5: F9, Lane 6: zebrafish brain, Lane 7: Hela.
2. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-β-tubulin antibody. Counter stained with hematoxylin.
3. ICC staining β-tubulin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
4. ICC stainingβ-tubulin in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. ICC staining:-tubulin in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. Flow cytometric analysis of HeLa?cells with β-tubulin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.

WB: 1:5000-10000; IHC: 1:20; ICC: 1:200; FCM: 1:50-100

Monoclonal

Unconjugated

Peptide