Anti-TTF1/NKX2-1 Antibody (4A120) is a Rabbit antibody targeting TTF1/NKX2-1. Anti-TTF1/NKX2-1 Antibody (4A120) can be used in FCM, ICC/IF, IF, IHC-Fr, IHC-P, WB.

IgG

4A120

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TTF1/NKX2-1 Monoclonal Antibody, Unconjugated (TMAB-13885) at 1:500 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Rat Thyroid Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TTF1/NKX2-1 Monoclonal Antibody, Unconjugated (TMAB-13885) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Thyroid Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TTF1/NKX2-1 Monoclonal Antibody, Unconjugated (TMAB-13885) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. (Negative control) Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TTF1/NKX2-1 Monoclonal Antibody, Unconjugated (TMAB-13885) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
5. 25 μg total protein per lane of various lysates (see on figure) probed with TTF1/NKX2-1 monoclonal antibody, unconjugated (TMAB-13885) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
6. Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TTF1/NKX2-1 Monoclonal Antibody, Unconjugated (TMAB-13885) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TTF1/NKX2-1 Monoclonal Antibody, Unconjugated (TMAB-13885) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
8. The TT (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-TTF1/NKX2-1 antibody (TMAB-13885): 1 μg/10^6 cells; Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
9. 4% Paraformaldehyde-fixed TT (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (TTF1/NKX2-1) monoclonal Antibody, unconjugated (TMAB-13885) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
10. Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TF1/NKX2-1 Monoclonal Antibody, Unconjugated (TMAB-13885) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.

FCM=1 μg/Test; ICC/IF=1:50-200; IF=1:100-500; IHC-Fr=1:100-500; IHC-P=1:100-500; WB=1:500-2000

Monoclonal

A synthesized peptide: human Nkx 2.1

Human

NKX2-1

Homeobox protein Nkx-2.1

Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis.