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Anti-TSG101 Antibody (9U262)
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Catalog No. TMAC-04144
Anti-TSG101 Antibody (9U262) is a Rabbit antibody targeting TSG101. Anti-TSG101 Antibody (9U262) can be used in FCM,ICC/IF,IHC,WB.
Anti-TSG101 Antibody (9U262)
Anti-TSG101 Antibody (9U262)
😃Good
Catalog No. TMAC-04144
Anti-TSG101 Antibody (9U262) is a Rabbit antibody targeting TSG101. Anti-TSG101 Antibody (9U262) can be used in FCM,ICC/IF,IHC,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
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| Description | Anti-TSG101 Antibody (9U262) is a Rabbit antibody targeting TSG101. Anti-TSG101 Antibody (9U262) can be used in FCM,ICC/IF,IHC,WB. |
| Ig Type | IgG |
| Clone | 9U262 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of TSG101 on different lysates using anti-TSG101 antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3, Lane 2: Hela, Lane 3: K562, Lane 4: Jurkat, Lane 5: A431. 2. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TSG101 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-TSG101 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TSG101 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-TSG101 antibody. Counter stained with hematoxylin. 6. ICC staining TSG101 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining TSG101 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining TSG101 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. Flow cytometric analysis of K562 cells with TSG101 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:100-500; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The transformation of a normal cell to one that is malignant can result from mutations in genes that encode proteins with key regulatory functions. Examples include the retinoblastoma gene product (Rb p110), p53, VHL and APC. Using a novel cloning strategy that allows the isolation of previously uncharacterized genes encoding selectable recessive phenotypes, an additional tumor suppressor gene has been identified. This gene, termed tsg 101 for tumor susceptibility gene 101, encodes a stathmin binding domain protein. When expression of this growth inhibitory gene is blocked in NIH/3T3 cells using antisense mRNA, the cells exhibit a transformed phenotype and are tumorigenic in SL6 mice. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 44 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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