Anti-Triosephosphate isomerase Polyclonal Antibody is a Rabbit antibody targeting Triosephosphate isomerase. Anti-Triosephosphate isomerase Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep)

1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-TPIS Polyclonal Antibody, Unconjugated (TMAB-13809) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample: Muscle (Mouse) Lysate at 40 μg
Primary: Anti-Triosephosphate isomerasee (TMAB-13809) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 30 kD
3. Sample: 293T (Human) Cell Lysate at 30 μg
Primary: Anti-Triosephosphate isomerase (TMAB-13809) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 27 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Triosephosphate isomerase

Human

TPI1

Triosephosphate isomerase