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Anti-Triosephosphate isomerase Polyclonal Antibody

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Catalog No. TMAB-13809

Anti-Triosephosphate isomerase Polyclonal Antibody is a Rabbit antibody targeting Triosephosphate isomerase. Anti-Triosephosphate isomerase Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-Triosephosphate isomerase Polyclonal Antibody

Anti-Triosephosphate isomerase Polyclonal Antibody

Copy Product Info
😃Good
Catalog No. TMAB-13809
Anti-Triosephosphate isomerase Polyclonal Antibody is a Rabbit antibody targeting Triosephosphate isomerase. Anti-Triosephosphate isomerase Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2237-10 days7-10 days
100 μL$3717-10 days7-10 days
200 μL$5287-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-Triosephosphate isomerase Polyclonal Antibody is a Rabbit antibody targeting Triosephosphate isomerase. Anti-Triosephosphate isomerase Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep)
Verified Activity
1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-TPIS Polyclonal Antibody, Unconjugated (TMAB-13809) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample: Muscle (Mouse) Lysate at 40 μg
Primary: Anti-Triosephosphate isomerasee (TMAB-13809) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 30 kD
3. Sample: 293T (Human) Cell Lysate at 30 μg
Primary: Anti-Triosephosphate isomerase (TMAB-13809) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 27 kD
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThis gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Triosephosphate isomerase
Antigen Species
Human
Gene Name
TPI1
Gene ID
Protein Name
Triosephosphate isomerase
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 27 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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