Anti-TRIM32 Polyclonal Antibody is a Rabbit antibody targeting TRIM32. Anti-TRIM32 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.

IgG

Mouse (predicted:Human,Rat)

1. Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TRIM32) Polyclonal Antibody, Unconjugated (TMAB-13781) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Blank control (Black line): Mouse spleen (Black).
Primary Antibody (green line): Rabbit Anti-TRIM32 antibody (TMAB-13781)
Dilution: 3 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 10,000 events was performed.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 3μg/Test

Polyclonal

KLH conjugated synthetic peptide: mouse TRIM32

Mouse

TRIM32

E3 ubiquitin-protein ligase TRIM32

Has an E3 ubiquitin ligase activity. Ubiquitinates DTNBP1 (dysbindin) (By similarity). Ubiquitinates PIAS4/PIASY and promotes its degradation in keratinocytes treated with UVB and TNF-alpha.