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Anti-TRIM14 Polyclonal Antibody

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Catalog No. TMAB-13768

Anti-TRIM14 Polyclonal Antibody is a Rabbit antibody targeting TRIM14. Anti-TRIM14 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-TRIM14 Polyclonal Antibody

Anti-TRIM14 Polyclonal Antibody

Copy Product Info
😃Good
Catalog No. TMAB-13768
Anti-TRIM14 Polyclonal Antibody is a Rabbit antibody targeting TRIM14. Anti-TRIM14 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2217-10 days7-10 days
100 μL$3747-10 days7-10 days
200 μL$5277-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-TRIM14 Polyclonal Antibody is a Rabbit antibody targeting TRIM14. Anti-TRIM14 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Dog,Pig,Rabbit)
Verified Activity
1. Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Mouse Spleen tissue lysates
Primary: Anti-TRIM14 (TMAB-13768) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
2. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (TRIM14) Polyclonal Antibody, Unconjugated (TMAB-13768) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Tissue SpecificityHighest expression in liver; undetectable in skeletal muscle.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThe protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies and its function has not been determined. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human TRIM14
Antigen Species
Human
Gene Name
TRIM14
Gene ID
Protein Name
Tripartite motif-containing protein 14
Uniprot ID
Function
Inhibits the transcriptional activity of SPI1 in a dose-dependent manner.
Chemical Properties
Molecular WeightTheoretical: 50 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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