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Anti-TRF1 Polyclonal Antibody
😃Good
Catalog No. TMAB-01894
Alias t-TRF1, TRF1, TRF, TRBF1, telomeric repeat binding factor (NIMA-interacting) 1, PIN2, hTRF1-AS, FLJ41416
Anti-TRF1 Polyclonal Antibody is a Rabbit antibody targeting TRF1. Anti-TRF1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-TRF1 Polyclonal Antibody
Anti-TRF1 Polyclonal Antibody
😃Good
Catalog No. TMAB-01894Alias t-TRF1, TRF1, TRF, TRBF1, telomeric repeat binding factor (NIMA-interacting) 1, PIN2, hTRF1-AS, FLJ41416
Anti-TRF1 Polyclonal Antibody is a Rabbit antibody targeting TRF1. Anti-TRF1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-TRF1 Polyclonal Antibody is a Rabbit antibody targeting TRF1. Anti-TRF1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | t-TRF1, TRF1, TRF, TRBF1, telomeric repeat binding factor (NIMA-interacting) 1, PIN2, hTRF1-AS, FLJ41416 |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TRF1) Polyclonal Antibody, Unconjugated (TMAB-01894) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 2. Sample: K562 (Human) Cell Lysate at 30 μg Primary: Anti-TRF1 (TMAB-01894) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kDa Observed band size: 49 kDa 3. Sample: HL-60 (Human) Cell Lysate at 30 μg Primary: Anti-TRF1 (TMAB-01894) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kDa Observed band size: 49 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. Cytoplasm; cytoskeleton; spindle. Chromosome; telomere. Colocalizes with telomeric DNA in interphase and metaphase cells and is located at chromosome ends during metaphase. Associates with the mitotic spindle. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes a telomere specific protein which is a component of the telomere nucleoprotein complex. This protein is present at telomeres throughout the cell cycle and functions as an inhibitor of telomerase, acting in cis to limit the elongation of individual chromosome ends. The protein structure contains a C-terminal Myb motif, a dimerization domain near its N-terminus and an acidic N-terminus. Two transcripts of this gene are alternatively spliced products. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH conjugated synthetic peptide: human TRBF1 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Telomeres |
| Molecular Weight | Theoretical: 50 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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