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Anti-TRF1 Polyclonal Antibody

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Catalog No. TMAB-01894
Alias t-TRF1, TRF1, TRF, TRBF1, telomeric repeat binding factor (NIMA-interacting) 1, PIN2, hTRF1-AS, FLJ41416

Anti-TRF1 Polyclonal Antibody is a Rabbit antibody targeting TRF1. Anti-TRF1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-TRF1 Polyclonal Antibody

Anti-TRF1 Polyclonal Antibody

😃Good
Catalog No. TMAB-01894Alias t-TRF1, TRF1, TRF, TRBF1, telomeric repeat binding factor (NIMA-interacting) 1, PIN2, hTRF1-AS, FLJ41416
Anti-TRF1 Polyclonal Antibody is a Rabbit antibody targeting TRF1. Anti-TRF1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2227-10 days
100 μL$3737-10 days
200 μL$5287-10 days
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Product Introduction

Bioactivity
Description
Anti-TRF1 Polyclonal Antibody is a Rabbit antibody targeting TRF1. Anti-TRF1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Aliast-TRF1, TRF1, TRF, TRBF1, telomeric repeat binding factor (NIMA-interacting) 1, PIN2, hTRF1-AS, FLJ41416
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TRF1) Polyclonal Antibody, Unconjugated (TMAB-01894) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
2. Sample:
K562 (Human) Cell Lysate at 30 μg
Primary: Anti-TRF1 (TMAB-01894) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 49 kDa
3. Sample:
HL-60 (Human) Cell Lysate at 30 μg
Primary: Anti-TRF1 (TMAB-01894) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 49 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus. Cytoplasm; cytoskeleton; spindle. Chromosome; telomere. Colocalizes with telomeric DNA in interphase and metaphase cells and is located at chromosome ends during metaphase. Associates with the mitotic spindle.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes a telomere specific protein which is a component of the telomere nucleoprotein complex. This protein is present at telomeres throughout the cell cycle and functions as an inhibitor of telomerase, acting in cis to limit the elongation of individual chromosome ends. The protein structure contains a C-terminal Myb motif, a dimerization domain near its N-terminus and an acidic N-terminus. Two transcripts of this gene are alternatively spliced products. [provided by RefSeq, Jul 2008]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human TRBF1
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Telomeres
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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