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Anti-TREM-1 Polyclonal Antibody
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Catalog No. TMAB-01891
Anti-TREM-1 Polyclonal Antibody is a Rabbit antibody targeting TREM-1. Anti-TREM-1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Anti-TREM-1 Polyclonal Antibody
Anti-TREM-1 Polyclonal Antibody
😃Good
Catalog No. TMAB-01891
Anti-TREM-1 Polyclonal Antibody is a Rabbit antibody targeting TREM-1. Anti-TREM-1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF,FCM Reactivity: Human,Rat |
| Ig Type | IgG |
| Reactivity | Human,Rat |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TREM1) Polyclonal Antibody, Unconjugated (TMAB-01891) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 2. Primary Antibody: Rabbit anti-TREM1 antibody Supplier catalog number: TMAB-01891 Supplier: Bioss Isotype Control Antibody: Rabbit IgG Secondary Antibody: f (ab/)2 fragment goat anti-rabbit IgG-PE Supplier: Jackson Lab. Positive control: U937 (4% paraformaldehyde for 10 min at room temperature) 3. Sample: hcclm3 (Human) Cell Lysate at 30 μg K562 (Human) Cell Lysate at 30 μg Primary: Anti-TREM1 (TMAB-01891) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kDa Observed band size: 21 kDa 4. Primary Antibody: Rabbit anti-TREM1 antibody Supplier catalog number: TMAB-01891 Supplier: Bioss Isotype Control Antibody: Rabbit IgG Secondary Antibody: f (ab/)2 fragment goat anti-rabbit IgG-PE Supplier: Jackson Lab. Positive control: U937 (4% paraformaldehyde for 10 min at room temperature) |
| Application | WB,IHC-P,IHC-Fr,IF,FCM |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Isoform 1: Cell membrane; Single-pass type I membrane protein (Potential). Isoform 2: Secreted (Potential). |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes a receptor belonging to the Ig superfamily that is expressed on myeloid cells. This protein amplifies neutrophil and monocyte-mediated inflammatory responses triggered by bacterial and fungal infections by stimulating release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Jun 2011]. |
| Immunogen | KLH conjugated synthetic peptide: human TREM1 |
| Antigen Species | Human |
| Gene ID | 54210 |
| Uniprot ID | |
| Biology Area | Macrophage / Inflamm. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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