Anti-TRAP80 Polyclonal Antibody is a Rabbit antibody targeting TRAP80. Anti-TRAP80 Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Mouse (predicted:Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep)

1. Blank control (blue line): Hela (fixed with pre-warmed 4% paraformaldehyde for 30 min at 37℃ and then permeabilized with 90% ice-cold methanol for 30 min on ice)
Primary Antibody (green line): Rabbit Anti-TRAP80 antibody (TMAB-13743),ilution: 0.2 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 μg /test.
2. Sample: MDA-MB-231 Cell (Human) Lysate at 40 μg
Primary: Anti-TRAP80 (TMAB-13743) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 73 kD
Observed band size: 73 kD
3. Sample: spleen (mouse) Lysate at 40 μg
Primary: Anti-TRAP80 (TMAB-13743) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 73 kD
Observed band size: 75 kD

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human TRAP80

Human

MED17

Mediator of RNA polymerase II transcription subunit 17

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.