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Anti-TRAP1/HSP75 Antibody (9U137)
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Catalog No. TMAB-13742
Anti-TRAP1/HSP75 Antibody (9U137) is a Rabbit antibody targeting TRAP1/HSP75. Anti-TRAP1/HSP75 Antibody (9U137) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Anti-TRAP1/HSP75 Antibody (9U137)
Anti-TRAP1/HSP75 Antibody (9U137)
Copy Product Info😃Good
Catalog No. TMAB-13742
Anti-TRAP1/HSP75 Antibody (9U137) is a Rabbit antibody targeting TRAP1/HSP75. Anti-TRAP1/HSP75 Antibody (9U137) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $151 | 7-10 days | 7-10 days | |
| 50 μL | $263 | 7-10 days | 7-10 days | |
| 100 μL | $471 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-TRAP1/HSP75 Antibody (9U137) is a Rabbit antibody targeting TRAP1/HSP75. Anti-TRAP1/HSP75 Antibody (9U137) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF. |
| Ig Type | IgG |
| Clone | 9U137 |
| Reactivity | Human |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TRAP1/HSP75 Monoclonal Antibody, Unconjugated (TMAB-13742) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TRAP1/HSP75 Monoclonal Antibody, Unconjugated (TMAB-13742) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TRAP1/HSP75 Monoclonal Antibody, Unconjugated (TMAB-13742) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 4. 25 μg total protein per lane of various lysates (see on figure) probed with TRAP1/HSP75 monoclonal antibody, unconjugated (TMAB-13742) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; ICC/IF: 1:50-200; IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Mitochondrion. |
| Tissue Specificity | Found in skeletal muscle, liver, heart, brain, kidney, pancreas, lung and placenta. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| Research Background | Chaperone that expresses an ATPase activity. Involved in maintaining mitochondrial function and polarization, downstream of PINK1 and mitochondrial complex I. Is a negative regulator of mitochondrial respiration able to modulate the balance between oxidative phosphorylation and aerobic glycolysis. The impact of TRAP1 on mitochondrial respiration is probably mediated by modulation of mitochondrial SRC and inhibition of SDHA. |
| Immunogen | A synthesized peptide: human Hsp75 |
| Antigen Species | Human |
| Gene Name | TRAP1 |
| Gene ID | |
| Protein Name | Heat shock protein 75 kDa, mitochondrial |
| Uniprot ID | |
| Function | Chaperone that expresses an ATPase activity. |
| Molecular Weight | Theoretical: 80 kDa. Actual: 75 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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