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Anti-Transglutaminase 2 Antibody (7U519)
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Catalog No. TMAB-13735
Anti-Transglutaminase 2 Antibody (7U519) is a Rabbit antibody targeting Transglutaminase 2. Anti-Transglutaminase 2 Antibody (7U519) can be used in WB,IHC-P,IF,IHC-Fr.
Anti-Transglutaminase 2 Antibody (7U519)
Anti-Transglutaminase 2 Antibody (7U519)
Copy Product Info😃Good
Catalog No. TMAB-13735
Anti-Transglutaminase 2 Antibody (7U519) is a Rabbit antibody targeting Transglutaminase 2. Anti-Transglutaminase 2 Antibody (7U519) can be used in WB,IHC-P,IF,IHC-Fr.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $150 | 7-10 days | 7-10 days | |
| 50 μL | $262 | 7-10 days | 7-10 days | |
| 100 μL | $470 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-Transglutaminase 2 Antibody (7U519) is a Rabbit antibody targeting Transglutaminase 2. Anti-Transglutaminase 2 Antibody (7U519) can be used in WB,IHC-P,IF,IHC-Fr. |
| Ig Type | IgG |
| Clone | 7U519 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Transglutaminase 2 Monoclonal Antibody, Unconjugated (TMAB-13735) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Transglutaminase 2 Monoclonal Antibody, Unconjugated (TMAB-13735) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Transglutaminase 2 Monoclonal Antibody, Unconjugated (TMAB-13735) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Transglutaminase 2 Monoclonal Antibody, Unconjugated (TMAB-13735) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Transglutaminase 2 Monoclonal Antibody, Unconjugated (TMAB-13735) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Mouse Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Transglutaminase 2 Monoclonal Antibody, Unconjugated (TMAB-13735) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Rat Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Transglutaminase 2 Monoclonal Antibody, Unconjugated (TMAB-13735) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Transglutaminase 2 Monoclonal Antibody, Unconjugated (TMAB-13735) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 9. Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Transglutaminase 2 Monoclonal Antibody, Unconjugated (TMAB-13735) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 10. 25 μg total protein per lane of various lysates (see on figure) probed with Transglutaminase 2 monoclonal antibody, unconjugated (TMAB-13735) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. |
| Application | |
| Recommended Dose | WB: 1: 500- 2000; IHC-P: 1: 100- 500; IF: 1: 100- 500; IHC-Fr: 1: 100- 500 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | Affinity Purification |
| Appearance | Liquid |
| Formulation | PBS with 100 μg/ml BSA, 0.15% ProClin300 and 50% glycerol. |
| Research Background | Calcium-dependent acyltransferase that catalyzes the formation of covalent bonds between peptide-bound glutamine and various primary amines, such as gamma-amino group of peptide-bound lysine, or mono- and polyamines, thereby producing cross-linked or aminated proteins, respectively. Involved in many biological processes, such as bone development, angiogenesis, wound healing, cellular differentiation, chromatin modification and apoptosis. Acts as a protein-glutamine gamma-glutamyltransferase by mediating the cross-linking of proteins, such as ACO2, HSPB6, FN1, HMGB1, RAP1GDS1, SLC25A4/ANT1, SPP1 and WDR54. Under physiological conditions, the protein cross-linking activity is inhibited by GTP; inhibition is relieved by Ca(2+) in response to various stresses. When secreted, catalyzes cross-linking of proteins of the extracellular matrix, such as FN1 and SPP1 resulting in the formation of scaffolds. Plays a key role during apoptosis, both by (1) promoting the cross-linking of cytoskeletal proteins resulting in condensation of the cytoplasm, and by (2) mediating cross-linking proteins of the extracellular matrix, resulting in the irreversible formation of scaffolds that stabilize the integrity of the dying cells before their clearance by phagocytosis, thereby preventing the leakage of harmful intracellular components. |
| Immunogen | A synthesized peptide: human TGM2 |
| Antigen Species | Human |
| Gene Name | TGM2 |
| Gene ID | |
| Protein Name | Protein-glutamine gamma-glutamyltransferase 2 |
| Uniprot ID | |
| Function | Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. |
| Molecular Weight | Theoretical: 78 kDa. Actual: 78 kDa. |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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