Anti-Transglutaminase 2 Antibody (4P75) is a Mouse antibody targeting Transglutaminase 2. Anti-Transglutaminase 2 Antibody (4P75) can be used in ICC/IF, IF, IHC-Fr, IHC-P, WB.

IgG1

4P75

Human

1. 25 ug total protein per lane of various lysates (see on figure) probed with Transglutaminase 2 monoclonal antibody, unconjugated (TMAB-13734) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Transglutaminase 2 Monoclonal Antibody, Unconjugated (TMAB-13734) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Transglutaminase 2 Monoclonal Antibody, Unconjugated (TMAB-13734) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Transglutaminase 2 Monoclonal Antibody, Unconjugated (TMAB-13734) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Transglutaminase 2 Monoclonal Antibody, Unconjugated (TMAB-13734) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Transglutaminase 2 Monoclonal Antibody, Unconjugated (TMAB-13734) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
7. 4% Paraformaldehyde-fixed HUVEC (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (Transglutaminase 2) monoclonal Antibody, unconjugated 1: 100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Mouse IgG antibody (green, BF488) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

ICC/IF=1:50-200; IF=1:200-800; IHC-Fr=1:200-800; IHC-P=1:200-800; WB=1:500-2000

Monoclonal

Recombinant Protein: Human TGM2 protein

Human

TGM2

Protein-glutamine gamma-glutamyltransferase 2

Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.