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Anti-Transferrin Receptor/TFRC Polyclonal Antibody

😃Good
Catalog No. TMAB-01887
Alias TR, TFRC, TfR1, TFR, T9, sTfR, p90, CD71

Anti-Transferrin Receptor/TFRC Polyclonal Antibody is a Rabbit antibody targeting Transferrin Receptor/TFRC. Anti-Transferrin Receptor/TFRC Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

Anti-Transferrin Receptor/TFRC Polyclonal Antibody

Anti-Transferrin Receptor/TFRC Polyclonal Antibody

😃Good
Catalog No. TMAB-01887Alias TR, TFRC, TfR1, TFR, T9, sTfR, p90, CD71
Anti-Transferrin Receptor/TFRC Polyclonal Antibody is a Rabbit antibody targeting Transferrin Receptor/TFRC. Anti-Transferrin Receptor/TFRC Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2217-10 days
100 μL$3727-10 days
200 μL$5277-10 days
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Product Introduction

Bioactivity
Description
Anti-Transferrin Receptor/TFRC Polyclonal Antibody is a Rabbit antibody targeting Transferrin Receptor/TFRC. Anti-Transferrin Receptor/TFRC Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
AliasTR, TFRC, TfR1, TFR, T9, sTfR, p90, CD71
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Dog,Pig,Cow,Horse,GuineaPig)
Verified Activity
1. Sample: Placenta (Mouse) Lysate at 40 μg
Primary: Anti-CD71/Transferrin receptor (TMAB-01887) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 74/85 kDa
Observed band size: 75 kDa
2. Blank control (blue): Raji (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-CD71 antibody (TMAB-01887), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit Igg (orange),used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
3. Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TFRC) Polyclonal Antibody, Unconjugated (TMAB-01887) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Blank control: K562. Primary Antibody (green line): Rabbit Anti-CD71 antibody (TMAB-01887)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
Application
WB,IHC-P,IHC-Fr,IF,FCM
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:50-200; FCM: 1μg /Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane; Single-pass type II membrane protein. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Transferrin receptor
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Response to hypoxia,Serum Proteins,Non-lineage,Hypoxia,B Lymphocytic Lineage,T Lymphocytic Lineage,Erythrocytic Lineage,Surface Molecules
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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