Anti-Transferrin Receptor/TFRC Polyclonal Antibody is a Rabbit antibody targeting Transferrin Receptor/TFRC. Anti-Transferrin Receptor/TFRC Polyclonal Antibody can be used in FCM, IF, IHC-Fr, IHC-P, WB.

IgG

Human,Mouse (predicted:Rat,Dog,Pig,Cow,Horse,GuineaPig)

1. Sample: Placenta (Mouse) Lysate at 40 μg
Primary: Anti-CD71/Transferrin receptor (TMAB-01887) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 74/85 kDa
Observed band size: 75 kDa
2. Blank control (blue): Raji (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-CD71 antibody (TMAB-01887), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit Igg (orange),used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
3. Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TFRC) Polyclonal Antibody, Unconjugated (TMAB-01887) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Blank control: K562. Primary Antibody (green line): Rabbit Anti-CD71 antibody (TMAB-01887)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

FCM=1 μg/Test; IF=1:100-500; IHC-Fr=1:100-500; IHC-P=1:100-500; WB=1:500-2000

Polyclonal

KLH conjugated synthetic peptide: human Transferrin receptor

Human

TFRC

Transferrin receptor protein 1

Response to hypoxia,Serum Proteins,Non-lineage,Hypoxia,B Lymphocytic Lineage,T Lymphocytic Lineage,Erythrocytic Lineage,Surface Molecules

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferring for an overlapping C-terminal binding site.