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Anti-Transferrin Receptor/TFRC Antibody (5J722)
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Catalog No. TMAY-01477
Alias transferrin receptor
Anti-Transferrin Receptor/TFRC Antibody (5J722) is a Rabbit antibody targeting Transferrin Receptor/TFRC. Anti-Transferrin Receptor/TFRC Antibody (5J722) can be used in IHC-P,IP.
Anti-Transferrin Receptor/TFRC Antibody (5J722)
Anti-Transferrin Receptor/TFRC Antibody (5J722)
😃Good
Catalog No. TMAY-01477Alias transferrin receptor
Anti-Transferrin Receptor/TFRC Antibody (5J722) is a Rabbit antibody targeting Transferrin Receptor/TFRC. Anti-Transferrin Receptor/TFRC Antibody (5J722) can be used in IHC-P,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $269 | 7-10 days |
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| Description | Anti-Transferrin Receptor/TFRC Antibody (5J722) is a Rabbit antibody targeting Transferrin Receptor/TFRC. Anti-Transferrin Receptor/TFRC Antibody (5J722) can be used in IHC-P,IP. |
| Alias | transferrin receptor |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 5J722 |
| Reactivity | Human |
| Verified Activity | 1. Immunochemical staining of human TFRC in human placenta with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to trophoblast. 2. Immunochemical staining of human TFRC in human colon carcinoma with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to intestinal gland. 3. TFRC was immunoprecipitated using: -Lane A:0.5 mg Jurkat Whole Cell Lysate. -Lane B:0.5 mg MCF-7 Whole Cell Lysate. -Lane C:0.5 mg HepG2 Whole Cell Lysate. -Lane D:0.5 mg Hela Whole Cell Lysate. -2 µL anti-TFRC rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-TFRC rabbit monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 84 kDa. -Observed band size: 100 kDa |
| Application | IHC-P,IP |
| Recommended Dose | IHC-P: 1:100-1:500; IP: 0.5-2 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human Transferrin Receptor / TFRC / CD71 (rh Transferrin Receptor / TFRC / CD71; TMPY-01873; AAA61153.1; Cys89-Phe760). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Transferrin receptor protein 1, also known as transferrin receptor, Trfr, p9, CD71 and TFRC, is a single-pass type II membrane protein that belongs to the peptidase M28 family and M28B subfamily. TFRC / CD71 is a membrane-bound protein expressed in larger amounts in proliferating. The specific expression of TFRC can represent a diagnostic tool or a therapeutic target in solid tumours expressing this antigen. Transferrin receptor is necessary for development of erythrocytes and the nervous system. TFRC / CD71 is regulated by cellular iron levels through binding of the iron regulatory proteins, IRP1 and IRP2, to iron-responsive elements in the 3'-UTR. Up-regulated upon mitogenic stimulation. TFRC / CD71 represents a marker of malignant transformation in the pancreas that could be applied as potential diagnostic and therapeutic target. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human Transferrin Receptor / TFRC / CD71 protein (TMPY-01873) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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