Anti-Transferrin Antibody (2P804) is a Mouse antibody targeting Transferrin. Anti-Transferrin Antibody (2P804) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

2P804

Human,Rat

1. Sample:
Lane 1: Serum (Human) at 20 μg
Primary: Anti-Transferrin (TMAB-13728) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 77 kD
Observed band size: 77 kD
2. Sample:
Lane 1: Serum (Rat) at 40 μg
Lane 2: Plasma (Rat) at 40 μg
Primary: Anti-Transferrin (TMAB-13728) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti--Mouse IgG at 1/20000 dilution
Predicted band size: 77 kD
Observed band size: 75 kD
3. Paraformaldehyde-fixed, paraffin embedded (Human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Transferrin) Monoclonal Antibody, Unconjugated (ascites of TMAB-13728 12D9) at 1: 2000 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Monoclonal

Full length native Transferrin protein purified from human plasma

Human

TF

Serotransferrin

Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.