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Anti-TRAIL/TNFSF10 Polyclonal Antibody
😃Good
Catalog No. TMAB-01886
Alias tumor necrosis factor (ligand) superfamily, member 10, Trail, TL2, Ly81, APO-2L, AI448571, A330042I21Rik
Anti-TRAIL/TNFSF10 Polyclonal Antibody is a Rabbit antibody targeting TRAIL/TNFSF10. Anti-TRAIL/TNFSF10 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-TRAIL/TNFSF10 Polyclonal Antibody
Anti-TRAIL/TNFSF10 Polyclonal Antibody
😃Good
Catalog No. TMAB-01886Alias tumor necrosis factor (ligand) superfamily, member 10, Trail, TL2, Ly81, APO-2L, AI448571, A330042I21Rik
Anti-TRAIL/TNFSF10 Polyclonal Antibody is a Rabbit antibody targeting TRAIL/TNFSF10. Anti-TRAIL/TNFSF10 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-TRAIL/TNFSF10 Polyclonal Antibody is a Rabbit antibody targeting TRAIL/TNFSF10. Anti-TRAIL/TNFSF10 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | tumor necrosis factor (ligand) superfamily, member 10, Trail, TL2, Ly81, APO-2L, AI448571, A330042I21Rik |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Sample: Lung (Mouse) Lysate at 30 μg U251 Cell Lysate at 30 μg Primary: rabbit Anti-TRAIL (TMAB-01886) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-rabbit Igg (secondary antibody) at 1:5000 dilution; Predicted band size: 31 kDa Observed band size: 26 kDa 2. Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-TRAIL Polyclonal Antibody, Unconjugated (TMAB-01886) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Tissue/cell: mouse spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-TRAIL Polyclonal Antibody, Unconjugated (TMAB-01886) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane; Single-pass type II membrane protein (Potential). |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. This protein binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3.(tumor necrosis factor-related apoptosis-inducing ligand) |
| Immunogen | KLH conjugated synthetic peptide: human TRAIL |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | TRAIL,TRAIL,Apoptosis,Death Ligands |
| Molecular Weight | Theoretical: 31 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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