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Anti-TRAIL/TNFSF10 Polyclonal Antibody

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Catalog No. TMAB-01886

Anti-TRAIL/TNFSF10 Polyclonal Antibody is a Rabbit antibody targeting TRAIL/TNFSF10. Anti-TRAIL/TNFSF10 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-TRAIL/TNFSF10 Polyclonal Antibody

Anti-TRAIL/TNFSF10 Polyclonal Antibody

😃Good
Catalog No. TMAB-01886
Anti-TRAIL/TNFSF10 Polyclonal Antibody is a Rabbit antibody targeting TRAIL/TNFSF10. Anti-TRAIL/TNFSF10 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$222 7-10 days
100 μL$374 7-10 days
200 μL$527 7-10 days
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Product Introduction

Bioactivity
Description
Anti-TRAIL/TNFSF10 Polyclonal Antibody is a Rabbit antibody targeting TRAIL/TNFSF10. Anti-TRAIL/TNFSF10 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse,Rat
Verified Activity
1. Sample:
Lung (Mouse) Lysate at 30 μg
U251 Cell Lysate at 30 μg
Primary: rabbit Anti-TRAIL (TMAB-01886) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit Igg (secondary antibody) at 1:5000 dilution;
Predicted band size: 31 kDa
Observed band size: 26 kDa
2. Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-TRAIL Polyclonal Antibody, Unconjugated (TMAB-01886) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Tissue/cell: mouse spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-TRAIL Polyclonal Antibody, Unconjugated (TMAB-01886) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationMembrane; Single-pass type II membrane protein (Potential).
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. This protein binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3.(tumor necrosis factor-related apoptosis-inducing ligand)
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human TRAIL
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
TRAIL,TRAIL,Apoptosis,Death Ligands
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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