Anti-TRAF2 Polyclonal Antibody is a Rabbit antibody targeting TRAF2. Anti-TRAF2 Polyclonal Antibody can be used in WB,FCM.

IgG

Human (predicted:Mouse,Rat)

1. Blank control: U937 (blue). Primary Antibody: Rabbit Anti-TRAF2 antibody (TMAB-01882), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange),used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (TMAB-01882, 1 μg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice.
2. Sample:
293t (Human) Cell Lysate at 30 μg
Primary: Anti-TRAF2 (TMAB-01882) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa
3. Sample: Hela Cell (Human) Lysate at 40 μg
Primary: Anti-TRAF2 (TMAB-01882) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa

WB,FCM

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human TRAF2

Human

TNF,Signal transduction,Inflammatory mediators,Associated Proteins,Cytoplasmic,TNF,NFkB Pathway