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Anti-TRAF2 Antibody (6B229)
😃Good
Catalog No. TMAC-04097
Alias Tumor necrosis factor type 2 receptor-associated protein 3, TRAP3, TRAF 2, TNF receptor-associated factor 2, RING-type E3 ubiquitin transferase TRAF2, EC 2.3.2.27, E3 ubiquitin-protein ligase TRAF2
Anti-TRAF2 Antibody (6B229) is a Rabbit antibody targeting TRAF2. Anti-TRAF2 Antibody (6B229) can be used in WB,ICC/IF,IHC,IP,FCM.
Anti-TRAF2 Antibody (6B229)
Anti-TRAF2 Antibody (6B229)
😃Good
Catalog No. TMAC-04097Alias Tumor necrosis factor type 2 receptor-associated protein 3, TRAP3, TRAF 2, TNF receptor-associated factor 2, RING-type E3 ubiquitin transferase TRAF2, EC 2.3.2.27, E3 ubiquitin-protein ligase TRAF2
Anti-TRAF2 Antibody (6B229) is a Rabbit antibody targeting TRAF2. Anti-TRAF2 Antibody (6B229) can be used in WB,ICC/IF,IHC,IP,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
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Chemical Properties
| Description | Anti-TRAF2 Antibody (6B229) is a Rabbit antibody targeting TRAF2. Anti-TRAF2 Antibody (6B229) can be used in WB,ICC/IF,IHC,IP,FCM. |
| Synonyms | Tumor necrosis factor type 2 receptor-associated protein 3, TRAP3, TRAF 2, TNF receptor-associated factor 2, RING-type E3 ubiquitin transferase TRAF2, EC 2.3.2.27, E3 ubiquitin-protein ligase TRAF2 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of TRAF2 on Hela cells lysates using anti-TRAF2 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TRAF2 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-TRAF2 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-TRAF2 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-TRAF2 antibody. Counter stained with hematoxylin. 6. ICC staining TRAF2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining TRAF2 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining TRAF2 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. Flow cytometric analysis of Hela cells with TRAF2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Tumor necrosis factor (TNF)-activated cell signaling is mediated primarily through the TNF receptor 1 (TNF-R1) and, to a lesser extent, TNF-R2. Both TNF receptors are members of the expanding TNF receptor superfamily, which includes the FAS antigen and CD40. Potential insight into an understanding of TNF receptor-mediated signaling was provided by the identification of two related proteins, TRAF1 and TRAF2 (for TNF receptor-associated factors 1 and 2, respectively). Both function to form heterodimeric complexes and associate with the cytoplasmic domain of TNF-R2. A third member of this protein family, alternatively designated CD40 bp, CRAF1, LAP1 or TRAF3, has been identified and shown to associate with the cytoplasmic domain of CD40. The similarity between a specific region of TRAF3 with regions of TRAF1 and TRAF2 define a ??TRAF-C?? domain that is necessary and sufficient for CD40 binding and homodimerization. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 56 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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