Anti-TRADD Polyclonal Antibody is a Rabbit antibody targeting TRADD. Anti-TRADD Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Rabbit)

1. Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-TRADD Polyclonal Antibody, Unconjugated (TMAB-13713) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-TRADD Polyclonal Antibody, Unconjugated (TMAB-13713) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
3. Sample:
Lane1: Colon carcinoma (Human) Lysate at 30 μg
Lane2: Brain (Mouse) Lysate at 30 μg
Primary: Anti-TRADD (TMAB-13713) at 1: 200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG at 1: 3000 dilution;
Predicted band size: 35 kD
Observed band size: 34 kD
4. Sample:
JurkaT (Human) Cell Lysate at 30 μg
MCF-7 (Human) Cell Lysate at 30 μg
Primary: Anti-TRADD (TMAB-13713) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34 kD
5. Sample: Cerebrum (Mouse) Lysate at 40 μg
Primary: Anti-TRADD (TMAB-13713) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: the middle of mouse TRADD

Mouse

TRADD

Tumor necrosis factor receptor type 1-associated DEATH domain protein

Adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B.