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Anti-TPM1 Antibody (5W651)
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Catalog No. TMAC-04136
Alias Tropomyosin-1, Tropomyosin alpha-1 chain, TPMA, TPM1, Alpha-tropomyosin
Anti-TPM1 Antibody (5W651) is a Rabbit antibody targeting TPM1. Anti-TPM1 Antibody (5W651) can be used in WB,IHC,FCM.
Anti-TPM1 Antibody (5W651)
Anti-TPM1 Antibody (5W651)
😃Good
Catalog No. TMAC-04136Alias Tropomyosin-1, Tropomyosin alpha-1 chain, TPMA, TPM1, Alpha-tropomyosin
Anti-TPM1 Antibody (5W651) is a Rabbit antibody targeting TPM1. Anti-TPM1 Antibody (5W651) can be used in WB,IHC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
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| Description | Anti-TPM1 Antibody (5W651) is a Rabbit antibody targeting TPM1. Anti-TPM1 Antibody (5W651) can be used in WB,IHC,FCM. |
| Synonyms | Tropomyosin-1, Tropomyosin alpha-1 chain, TPMA, TPM1, Alpha-tropomyosin |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of Tropomyosin 1 on rat heart tissue using anti-Tropomyosin 1 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded rat smooth muscle tissue using anti-Tropomyosin 1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human heart tissue using anti-Tropomyosin 1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Tropomyosin 1 antibody. Counter stained with hematoxylin. 5. Flow cytometric analysis of MCF-7 cells with Tropomyosin 1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Tropomyosins are a group of structural proteins. Tropomyosins are present in virtually all eukaryotic cells, both muscle and non-muscle, where they bind Actin filaments and function to modulate Actin-Myosin interaction and stabilize Actin filament structure. Tropomyosin α is encoded by the TPM1 gene, which maps to human chromosome 15q22.1 and undergoes alternative splicing to generate at least four isoforms, including skeletal muscle (isoform 1), smooth muscle (isoform 2), fibroblast/TM3 (isoform 3) and isoform 4. Tropomyosin β is encoded by the TPM2 gene, which maps to human chromosome 9p13.3 and undergoes alternative splicing to generate three isoforms, including skeletal muscle (isoform 1), non-muscle/fibroblast TM36/epithelial TMe1 (isoform 2) and non-muscle (isoform 3). Troponin I binds Tropomyosin at a specific region and the association of Tropomyosin-Troponin with Actin filaments may increase the rigidity of Actin filaments. Tropomyosin also interacts with Caldesmon to regulate smooth muscle contraction. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: human Tropomyosin 1 aa 100-300 |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Theoretical: 33 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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