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Anti-TPH2 Polyclonal Antibody

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Catalog No. TMAB-01879
Alias Tryptophan 5-monooxygenase 2, Tryptophan 5-hydroxylase 2, TPH2, NTPH, Neuronal tryptophan hydroxylase

Anti-TPH2 Polyclonal Antibody is a Rabbit antibody targeting TPH2. Anti-TPH2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-TPH2 Polyclonal Antibody

Anti-TPH2 Polyclonal Antibody

😃Good
Catalog No. TMAB-01879Alias Tryptophan 5-monooxygenase 2, Tryptophan 5-hydroxylase 2, TPH2, NTPH, Neuronal tryptophan hydroxylase
Anti-TPH2 Polyclonal Antibody is a Rabbit antibody targeting TPH2. Anti-TPH2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2217-10 days
100 μL$3727-10 days
200 μL$5277-10 days
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Product Introduction

Bioactivity
Description
Anti-TPH2 Polyclonal Antibody is a Rabbit antibody targeting TPH2. Anti-TPH2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
AliasTryptophan 5-monooxygenase 2, Tryptophan 5-hydroxylase 2, TPH2, NTPH, Neuronal tryptophan hydroxylase
Ig Type
IgG
Reactivity
Human,Rat (predicted:Mouse,Cow,Horse)
Verified Activity
1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-TPH2 Polyclonal Antibody, Unconjugated (TMAB-01879) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample:
U87Mg (Human) Cell Lysate at 30 μg
U251 (Human) Cell Lysate at 30 μg
Primary: Anti-TPH2 (TMAB-01879) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 60 kDa
3. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TPH2) Polyclonal Antibody, Unconjugated (TMAB-01879) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundPhenylalanine hydroxylase (PAH), tyrosine hydroxylase (TH), tryptophan hydroxylase (TPH) and tryptophan hydroxylase 2 (TPH2) comprise a small family of monooxygenases that catalyze the rate-limiting step in the catabolism of aromatic L-amino acids and utilize tetrahydropterine as a cofactor. TPH2 is highly expressed in the central nervous system (CNS), mainly in the brain. TPH2 catalyzes the first step in the biosynthesis of serotonin in the CNS and melatonin in the pineal gland, and may be involved in the pathology of several neuropsychiatric disorders. Glucocorticoid-mediated reduction of TPH2 is associated with the etiology of mood disorders, specifically psychotic major depression, and TPH2 may be related to dysregulation of serotonin neurotransmission in the brain which commonly leads to suicidal behavior.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human TPH2
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Serotonin / 5HT
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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