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Anti-TPH Antibody (6K304)

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Catalog No. TMAB-13693

Anti-TPH Antibody (6K304) is a Rabbit antibody targeting TPH. Anti-TPH Antibody (6K304) can be used in WB, IHC-P, IHC-Fr, IP, IF.

Anti-TPH Antibody (6K304)

Anti-TPH Antibody (6K304)

Copy Product Info
😃Good
Catalog No. TMAB-13693
Anti-TPH Antibody (6K304) is a Rabbit antibody targeting TPH. Anti-TPH Antibody (6K304) can be used in WB, IHC-P, IHC-Fr, IP, IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
25 μL$1507-10 days7-10 days
50 μL$2617-10 days7-10 days
100 μL$4737-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-TPH Antibody (6K304) is a Rabbit antibody targeting TPH. Anti-TPH Antibody (6K304) can be used in WB, IHC-P, IHC-Fr, IP, IF.
Ig Type
IgG
Clone
6K304
Reactivity
Human,Mouse,Rat
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TPH Monoclonal Antibody, Unconjugated (TMAB-13693) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TPH Monoclonal Antibody, Unconjugated (TMAB-13693) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TPH Monoclonal Antibody, Unconjugated (TMAB-13693) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
4. 25 ug total protein per lane of various lysates (see on figure) probed with TPH monoclonal antibody, unconjugated (TMAB-13693) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
5. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TPH Monoclonal Antibody, Unconjugated (TMAB-13693) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, Goat Anti-Rabbit IgG H&L-BF594), DAPI (blue) was used to stain the cell nuclei.
6. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TPH Monoclonal Antibody, Unconjugated (TMAB-13693) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, Goat Anti-Rabbit IgG H&L-BF594), DAPI (blue) was used to stain the cell nuclei.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IP: 1:20-50; IF: 1:50-200
Antibody Type
Monoclonal
Host SpeciesRabbit
Tissue SpecificityIsoform 2 seems to be less widely expressed than isoform 1.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Research BackgroundOxidizes L-tryptophan to 5-hydroxy-l-tryptophan in the rate-determining step of serotonin biosynthesis.
Antigen Details
Immunogen
A synthesized peptide: human TPH1
Antigen Species
Human
Gene Name
TPH1
Gene ID
Protein Name
Tryptophan 5-hydroxylase 1
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 51 kDa. Actual: 51 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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