Shopping Cart
  • Remove All
  • TargetMol
    Your shopping cart is currently empty

Anti-TnTc Polyclonal Antibody

😃Good
Catalog No. TMAB-01877

Anti-TnTc Polyclonal Antibody is a Rabbit antibody targeting TnTc. Anti-TnTc Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.

Anti-TnTc Polyclonal Antibody

Anti-TnTc Polyclonal Antibody

😃Good
Catalog No. TMAB-01877
Anti-TnTc Polyclonal Antibody is a Rabbit antibody targeting TnTc. Anti-TnTc Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$222 7-10 days
100 μL$372 7-10 days
200 μL$529 7-10 days
Add to Cart
Add to Quotation
Bulk & Custom
Questions
View More
Contact us for more batch information
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Product Introduction

Bioactivity
Description
Anti-TnTc Polyclonal Antibody is a Rabbit antibody targeting TnTc. Anti-TnTc Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Dog,Pig,Cow)
Verified Activity
1. Sample:
H9C2 (Rat) Cell Lysate at 30 μg
Primary: Anti-alpha smooth muscle Actin (TMAB-01877) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
2. Blank control: U-2OS.
Primary Antibody (green line): Rabbit Anti-TNNT2 antibody (TMAB-01877)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
3. Cell: Neonatal rat ventricular cardiomyocytes; Dilution: 1:400; Incubation: Anti-Cardiac Troponin T Antibody, unconjugated (TMAB-01877); DAPI was used to stain the cell nucleus. The image is provided by Tongji University.
4. Positive control: H9C2 cells
Concebtration: 2 μg/10^6 cells
Incubation conditions: Avoid light , 30 minutes on the ice.
5. Tissue/cell: rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-TNNT2 Polyclonal Antibody, Unconjugated (TMAB-01877) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
6. Tissue/cell: rat heart tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-TNNT2 Polyclonal Antibody, Unconjugated 1:500, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml,blue) was used to stain the cell nucleus.
7. Paraformaldehyde-fixed, paraffin embedded (mouse heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cardiac Troponin T) Polyclonal Antibody, Unconjugated (TMAB-01877) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Application
WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500; FCM: 1μg/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq].
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Cardiac Troponin T
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Cardiomyocytes,Troponin,Troponin,Myogenesis
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

Calculator

  • Molarity Calculator
  • Dilution Calculator
  • Reconstitution Calculator
  • Molecular Weight Calculator

Sci Citations

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Related Tags: buy Anti-TnTc Polyclonal Antibody | purchase Anti-TnTc Polyclonal Antibody | Anti-TnTc Polyclonal Antibody cost | order Anti-TnTc Polyclonal Antibody