Anti-TNPO3 Antibody (6F570) is a Rabbit antibody targeting TNPO3. Anti-TNPO3 Antibody (6F570) can be used in ICC/IF, IF, IHC-Fr, IHC-P, WB.

IgG

6F570

Human,Mouse,Rat

1. 25 ug total protein per lane of various lysates (see on figure) probed with TNPO3 monoclonal antibody, unconjugated (TMAB-13655) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TNPO3 Monoclonal Antibody, Unconjugated (TMAB-13655) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TNPO3 Monoclonal Antibody, Unconjugated (TMAB-13655) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TNPO3 Monoclonal Antibody, Unconjugated (TMAB-13655) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TNPO3 Monoclonal Antibody, Unconjugated (TMAB-13655) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TNPO3 Monoclonal Antibody, Unconjugated (TMAB-13655) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TNPO3 Monoclonal Antibody, Unconjugated (TMAB-13655) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Rat Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TNPO3 Monoclonal Antibody, Unconjugated (TMAB-13655) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.

ICC/IF=1:50-200; IF=1:50-200; IHC-Fr=1:50-200; IHC-P=1:50-200; WB=1:500-2000

Monoclonal

A synthesized peptide: human Transportin 3

Human

Tnpo3

Transportin-3

Seems to function in nuclear protein import as nuclear transport receptor. In vitro, mediates the nuclear import of splicing factor SR proteins SFRS1 and SFRS2, by recognizing phosphorylated RS domains.