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Anti-TNNI3 Polyclonal Antibody
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Catalog No. TMAB-01876
Alias Ttroponin I type 3(cardiac), TroponinI, Troponin I, cardiac muscle, troponin I, cardiac 3, Troponin I type 3 cardiac, Troponin I cardiac muscle isoform, Troponin I cardiac muscle, Troponin I cardiac, Troponin I, TNNI3, TNNI 3, TNNC1, TNNC 1, TNN I3, Tni, Tn1, RCM1, MGC116817, Familial hypertrophic cardiomyopathy 7, cTnI, CMH7, CMD2A, CMD1FF, Cardiomyopathy, familial hypertrophic, 7, included, Cardiac Troponin I, cardiac muscle
Anti-TNNI3 Polyclonal Antibody is a Rabbit antibody targeting TNNI3. Anti-TNNI3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Anti-TNNI3 Polyclonal Antibody
Anti-TNNI3 Polyclonal Antibody
😃Good
Catalog No. TMAB-01876Alias Ttroponin I type 3(cardiac), TroponinI, Troponin I, cardiac muscle, troponin I, cardiac 3, Troponin I type 3 cardiac, Troponin I cardiac muscle isoform, Troponin I cardiac muscle, Troponin I cardiac, Troponin I, TNNI3, TNNI 3, TNNC1, TNNC 1, TNN I3, Tni, Tn1, RCM1, MGC116817, Familial hypertrophic cardiomyopathy 7, cTnI, CMH7, CMD2A, CMD1FF, Cardiomyopathy, familial hypertrophic, 7, included, Cardiac Troponin I, cardiac muscle
Anti-TNNI3 Polyclonal Antibody is a Rabbit antibody targeting TNNI3. Anti-TNNI3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-TNNI3 Polyclonal Antibody is a Rabbit antibody targeting TNNI3. Anti-TNNI3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM. |
| Alias | Ttroponin I type 3(cardiac), TroponinI, Troponin I, cardiac muscle, troponin I, cardiac 3, Troponin I type 3 cardiac, Troponin I cardiac muscle isoform, Troponin I cardiac muscle, Troponin I cardiac, Troponin I, TNNI3, TNNI 3, TNNC1, TNNC 1, TNN I3, Tni, Tn1, RCM1, MGC116817, Familial hypertrophic cardiomyopathy 7, cTnI, CMH7, CMD2A, CMD1FF, Cardiomyopathy, familial hypertrophic, 7, included, Cardiac Troponin I, cardiac muscle |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Blank control: K562. Primary Antibody (green line): Rabbit Anti-TNNI3 antibody (TMAB-01876) Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC Dilution: 1 μg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. 2. Sample: Lane 1: Heart (Mouse) Lysate at 40 μg Lane 2: Heart (Rat) Lysate at 40 μg Primary: Anti-TNNI3 (TMAB-01876) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28 kDa Observed band size: 28 kDa 3. Paraformaldehyde-fixed, paraffin embedded (mouse heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TNNI3) Polyclonal Antibody, Unconjugated (TMAB-01876) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TNNI3) Polyclonal Antibody, Unconjugated (TMAB-01876) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 5. Sample: heart (rat) lysate at 40 μg Primary: Anti-TNNI3 (TMAB-01876) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kDa Observed band size: 24 kDa 6. Sample: LympHnode (Rat) Cell Lysate at 40 μg Primary: Anti-TNNI3 (TMAB-01876) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kDa Observed band size: 24 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 2ug/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008] |
| Immunogen | KLH conjugated synthetic peptide: human TNNI3 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Troponin,Troponin |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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