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Anti-TNNI3 Antibody (4G587)
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Catalog No. TMAB-13653
Anti-TNNI3 Antibody (4G587) is a Rabbit antibody targeting TNNI3. Anti-TNNI3 Antibody (4G587) can be used in WB,IHC-P,IHC-Fr,IF.
Anti-TNNI3 Antibody (4G587)
Anti-TNNI3 Antibody (4G587)
Copy Product Info😃Good
Catalog No. TMAB-13653
Anti-TNNI3 Antibody (4G587) is a Rabbit antibody targeting TNNI3. Anti-TNNI3 Antibody (4G587) can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $149 | 7-10 days | 7-10 days | |
| 50 μL | $262 | 7-10 days | 7-10 days | |
| 100 μL | $473 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Bioactivity
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| Description | Anti-TNNI3 Antibody (4G587) is a Rabbit antibody targeting TNNI3. Anti-TNNI3 Antibody (4G587) can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Clone | 4G587 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded Mouse Embryo (heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TNNI3 Monoclonal Antibody, Unconjugated (TMAB-13653) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Rat Embryo (heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TNNI3 Monoclonal Antibody, Unconjugated (TMAB-13653) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TNNI3 Monoclonal Antibody, Unconjugated (TMAB-13653) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TNNI3 Monoclonal Antibody, Unconjugated (TMAB-13653) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TNNI3 Monoclonal Antibody, Unconjugated (TMAB-13653) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 6. 25 μg total protein per lane of various lysates (see on figure) probed with TNNI3 monoclonal antibody, unconjugated (TMAB-13653) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| Research Background | Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments. |
| Immunogen | A synthesized peptide: human TNNC1 |
| Antigen Species | Human |
| Gene Name | TNNC1 |
| Gene ID | |
| Protein Name | Troponin C, slow skeletal and cardiac muscles |
| Uniprot ID |
| Molecular Weight | Theoretical: 18 kDa. Actual: 18 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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