Anti-TNNI1 Antibody (4R883) is a Rabbit antibody targeting TNNI1. Anti-TNNI1 Antibody (4R883) can be used in IF, IHC-Fr, IHC-P, IP, WB.

IgG

4R883

Human

1. Western blot analysis of Human fetal heart lysate. Using TNNI1 (TMAB-13651) monoclonal antibody at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TNNI1 Monoclonal Antibody, Unconjugated (TMAB-13651) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
3. (Negative control) Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TNNI1 Monoclonal Antibody, Unconjugated (TMAB-13651) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.

IF=1:100-500; IHC-Fr=1:100-500; IHC-P=1:100-500; IP=1:20-50; WB=1:1000-2000

Monoclonal

A synthesized peptide: human TNNI1

Human

TNNI1

Troponin I, slow skeletal muscle

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.