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Anti-TMEM85 Polyclonal Antibody

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Catalog No. TMAB-13608

Anti-TMEM85 Polyclonal Antibody is a Rabbit antibody targeting TMEM85. Anti-TMEM85 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-TMEM85 Polyclonal Antibody

Anti-TMEM85 Polyclonal Antibody

Copy Product Info
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Catalog No. TMAB-13608
Anti-TMEM85 Polyclonal Antibody is a Rabbit antibody targeting TMEM85. Anti-TMEM85 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2237-10 days7-10 days
100 μL$3737-10 days7-10 days
200 μL$5297-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-TMEM85 Polyclonal Antibody is a Rabbit antibody targeting TMEM85. Anti-TMEM85 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Pig,Cow,Horse,Rabbit,Sheep)
Verified Activity
1. Sample: U251 (Human) Cell Lysate at 40 μg
Primary: Anti-TMEM85 (TMAB-13608) at 1/500 dilution
Anti-TMEM85 (TMAB-13608) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17 kD
Observed band size: 17 kD
2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TMEM85) Polyclonal Antibody, Unconjugated (TMAB-13608) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TMEM85) Polyclonal Antibody, Unconjugated (TMAB-13608) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Application
WB
IHC-P
IHC-Fr
IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationMembrane.
Tissue SpecificityIsoform 1 is expressed in brain and heart. Isoform 2 is expressed in heart.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundMay mediate anti-apoptotic activity.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human TMEM85
Antigen Species
Human
Gene Name
EMC4
Gene ID
51234
Protein Name
ER membrane protein complex subunit 4
Uniprot ID
Q5J8M3
Chemical Properties
Molecular WeightTheoretical: 20, 17 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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