Anti-TMEM67/Meckelin Antibody (6J757) is a Rabbit antibody targeting TMEM67/Meckelin. Anti-TMEM67/Meckelin Antibody (6J757) can be used in FCM, ICC/IF, WB.

IgG

6J757

Human,Mouse,Rat

1. 25 ug total protein per lane of various lysates (see on figure) probed with TMEM67/Meckelin monoclonal antibody, unconjugated (TMAB-13607) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. 4% Paraformaldehyde-fixed HepG2 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (TMEM67/Meckelin) monoclonal Antibody, unconjugated (TMAB-13607) 1: 100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, BF488) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

FCM=1:50-100; ICC/IF=1:50-200; WB=1:500-2000

Monoclonal

A synthesized peptide: human Meckelin

Human

TMEM67

Meckelin

Required for ciliary structure and function. Part of the tectonic-like complex which is required for tissue-specific ciliogenesis and may regulate ciliary membrane composition By similarity. Involved in centrosome migration to the apical cell surface during early ciliogenesis. Involved in the regulation of cilia length and appropriate number through the control of centrosome duplication. Required for cell branching morphology. Essential for endoplasmic reticulum-associated degradation (ERAD) of surfactant protein C (SFTPC).