Anti-TMEM176A Polyclonal Antibody is a Rabbit antibody targeting TMEM176A. Anti-TMEM176A Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse (predicted:Rat)

1. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TMEM176A) Polyclonal Antibody, Unconjugated (TMAB-13595) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Protein: HepG2 (human) lysates at 30 μg;
Primary: rabbit Anti-TMEM176A (TMAB-13595) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1: 5000;
Predicted band size: 26 kD
Observed band size: 26 kD
3. Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-TMEM176A Polyclonal Antibody, Unconjugated (TMAB-13595) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
4. Protein: mouse kidney lysates, 30 μg;
Primary: Anti-TMEM176A (TMAB-13595) at 1:300;
Secondary: HRP conjugated Goat Anti-Rabbit IgG at 1:5000;
ECL excitated the fluorescence;
Predicted band size: 24 kD
Observed band size: 24 kD
5. Sample: U937 (Human) Cell Lysate at 30 μg
Primary: Anti-TMEM176A (TMAB-13595) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kD
Observed band size: 25 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: MOUSE TMEM176A

Mouse

TMEM176A

Transmembrane protein 176A