Anti-TMEM158 Polyclonal Antibody is a Rabbit antibody targeting TMEM158. Anti-TMEM158 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.

IgG

Mouse,Rat (predicted:Human,Chicken,Pig,Cow)

1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-TMEM158 Polyclonal Antibody, Unconjugated (TMAB-13594) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TMEM158) Polyclonal Antibody, Unconjugated (TMAB-13594) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TMEM158) Polyclonal Antibody, Unconjugated (TMAB-13594) at 1:400 overnight at 4°C, followed by a conjugated secondary at [1:500] for 90 minutes and DAPI staining of the nuclei.
4. Blank control: Mouse heart cells (blue). Primary Antibody: Rabbit Anti-TMEM158 antibody (TMAB-13594), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1: 200 in 1 X PBS containing 0.5% BSA.
5. Blank control: Mouse spleen cells (blue). Primary Antibody: Rabbit Anti-TMEM158 antibody (TMAB-13594), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1: 200 in 1 X PBS containing 0.5% BSA.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human TMEM158

Human

TMEM158

Transmembrane protein 158

Receptor for brain injury-derived neurotrophic peptide (BINP), a synthetic 13-mer peptide.