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Anti-TMEFF2 Polyclonal Antibody
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Catalog No. TMAB-13584 Copy Product Info
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Anti-TMEFF2 Polyclonal Antibody is a Rabbit antibody targeting TMEFF2. Anti-TMEFF2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-TMEFF2 Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 µL | $221 | 7-10 days | 7-10 days | |
| 100 µL | $371 | 7-10 days | 7-10 days | |
| 200 µL | $528 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-TMEFF2 Polyclonal Antibody is a Rabbit antibody targeting TMEFF2. Anti-TMEFF2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit,GuineaPig) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TMEFF2) Polyclonal Antibody, Unconjugated (TMAB-13584) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (rat spinal tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TMEFF2) Polyclonal Antibody, Unconjugated (TMAB-13584) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 3. Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Rat Cerebrum tissue lysates Primary: Anti-TMEFF2 (TMAB-13584) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kDa Observed band size: 44 kDa 4. Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (TMEFF2) Polyclonal Antibody, Unconjugated (TMAB-13584) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Isoform 1: Membrane; Single-pass type I membrane protein (Potential). Isoform 2: Membrane; Single-pass type I membrane protein (Potential). Isoform 3: Secreted. |
| Tissue Specificity | Highly expressed in adult and fetal brain, spinal cord and prostate. Expressed in all brain regions except the pituitary gland, with highest levels in amygdala and corpus callosum. Expressed in the pericryptal myofibroblasts and other stromal cells of normal colonic mucosa. Expressed in prostate carcinoma. Down-regulated in colorectal cancer. Present in Alzheimer disease plaques (at protein level). Isoform 3 is expressed weakly in testis and at high levels in normal and cancerous prostate. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | TMEFF2 is a growth factor implicated in the progression of prostate cancer. It is highly expressed in the brain and may act as a survival factor for mesencephalic and hippocampal neurons. |
| Immunogen | KLH conjugated synthetic peptide: human TMEFF2 |
| Antigen Species | Human |
| Gene Name | TMEFF2 |
| Gene ID | |
| Protein Name | Tomoregulin-2 |
| Uniprot ID | |
| Function | May be a survival factor for hippocampal and mesencephalic neurons. The shedded form up-regulates cancer cell proliferation, probably by promoting ERK1/2 phosphorylation. |
| Molecular Weight | Theoretical: 37 kDa. Actual: 44 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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